
![]() The NEPHELOstar Plus microplate nephelometer detects insoluble particles in liquid samples by measuring forward scattered light. It uses a laser beam that is directed through the solution and it can detect the scattered light at angles of up to 80 degrees. The NEPHELOstar Plus can be up to thirty times more sensitive than traditional transmission readers that measure the reduction in direct light passing through a sample well via optical density.
Developed to meet high-throughput demands, this microplate-based nephelometer is faster than conventional solubility detection methods with the highest possible sensitivity and flexibility. The measurement of turbidity, which is the haziness or cloudiness of a fluid that sometimes cannot been seen by the naked eye, can be used to determine the amount of insoluble particles in a solution. Amongst the life science applications that can use turbidity measurements include: drug compound solubility, bacterial or fungal growth studies, immunoprecipitations, protein aggregation, antibody-antigen interaction, and polymerization monitoring. Turbidity measurements can also be performed with a nephelometric instrument that measures light-scattering, such as the NEPHELOstar Plus from BMG LABTECH. This instrument uses a diode laser that passes light through each sample. If there are insoluble particles in the solution, the laser light will be scattered by the particles. Similar to absorbance measurements, the amount of scattered light is directly proportional to the amount of particles in solution. However, light-scattering measurements do not suffer from the same upper limitation as absorbance measurements, thereby providing a wider dynamic range for turbidity measurements. The light source is a red laser with a wavelength range of 635 nm. A laser diode has the advantage of higher stability than other light sources which gives better precision. The laser intensity can be adjusted via the software. |
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| Detection Modes | Nephelometry; Light Scattering |
| Measurement Modes | Endpoint and Kinetic measurements |
| Microplate Formats | Up to 384-well plates |
| Microplate Carrier | Robot compatible |
| Light Sources | Self-monitoring Laser diode Wavelength 635±10 nm Stability <0.2% deviation Lifetime 20,000 hours Output: 1 mW Selectable beam width: 1.5 to 3.5 mm Selectable Intensity 0-100% Scattering angle: Detects up to 80° full cone angle |
| Detectors | Side window photodiode detector |
| Sensitivity* | Depends on particle size and liquid properties Silica detection (particle size 0.5 to 10 µm) 800 nM Dynamic Range: 5 decades Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second) |
| Read Times | Depend on assay conditions and liquid surface stability 16 s (96), 47 s (384) (shortest possible times) |
| Reagent Injection | Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 500 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing |
| Shaking | Linear, orbital, and double-orbital with user-definable time and speed |
| Purge Gas Vent | System to inject an atmosphere or to pull a vacuum into the reader |
| Incubation | +4°C above ambient up to 45°C |
| Software | Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant |
| Dimensions | Width: 44 cm, depth: 48 cm, height: 32 cm; weight: 25 kg |
| Accessories | Optional Accessories |
| Atmospheric Control Unit (ACU) | Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg |
| Stacker | Magazines for up to 50 plates - continuous loading feature |
| THERMOstar | Microplate Incubator and Shaker |
| Upgrades | Upgrades to include options such as reagent injectors, etc. are available. Please contact your local representative for more information. |
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
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