|UV/Vis absorbance spectra|
The SPECTROstar Nano represents a revolution in absorbance measurement instrumentation with the flexibility to perform assays quickly and easily in both microplates or via the built-in cuvette port. Using a spectrometer, this new absorbance microplate reader captures a full UV-Visible spectrum (220 to 1000 nm) in less than 1 sec/well and measures sample volumes down to 2 µL. The speed of the spectrometer, simple push button operation, and the capacity to design and save individual assay protocols give users an unmatched flexibility to optimize settings for all absorbance experiments.
The SPECTROstar Nano also includes the following outstanding features
- Ultra-fast UV/Vis absorbance spectrometer
- Captures a spectrum from 220 to 1000 nm in less than 1 s/well
- Selectable spectral resolution of 1 - 10 nm
- 6- to 1536-well microplate format compatibility
- Low volume 2 µL measurements for DNA, RNA, protein, etc.
- Standard cuvette port for individual samples
- Incubation up to 45°C for microplate and cuvette
- Gas vent for atmospheric sensitive samples
- Well scanning, kinetic and endpoint measurements
- Multimode plate shaking capabilities
- Assay specific quick run protocols
- Powerful Data Analysis Software - MARS
- FDA 21 CFR part 11 compliant
- Robot compatibility
- Much more...
The SPECTROstar Nano is delivered with a new version of BMG LABTECH's established Reader Control software and comprehensive Data Analysis Software, MARS. With its simple to use 'Quick Run' feature it reads ELISAs, RNA, DNA, Protein (Bradford, BCA, Lowry), Cell growth, and Beta-galactosidase assays at the touch of a button due to predefined protocols. This highly intuitive software also provides limitless possibilities for test set up where it is needed. In MARS, a wizard guides the user through the entire data analysing process. Additionally, a template manager can be used for building complex data processing protocols. Improved robotic integration capabilities, digital signature and FDA 21 CFR part 11 compliance of MARS complete this powerful software package.
SPECTROstar Nano applications include:
- 260/280 nm DNA quantitation
- Protein quantitation (e.g. Bradford Lowry, BCA)
- Reporter gene assays (e.g. ß-Gal, SEAP)
- Cell proliferation and apoptosis
- ELISA, enzyme immunoassays (e.g. alkaline phosphatase, horseradish peroxidase)
- Enzyme activity assays: Monitoring absorbance changes in cellular co-factors NADH and NADPH allowing the determination of enzymatic activity
- Microbial growth
- Protein aggregation
The speed of the spectrometer and the easy work-up in the software provide users with unmatched flexibility that can be used to optimize absorbance settings for all experiments.
Now Perform Label-Free SoPRano™ Assays Using Ultra-Fast, Full Spectrum Analysis
In collaboration with Pharma Diagnotics, all spectrometer-based microplate readers from BMG LABTECH have been tested and certified to be used with the label-free, Gold Nano Rods SoPRano™ assay . The SoPRano™ assay, which is based upon localized surface plasmon resonance (LSPR), allows for homogeneous label-free protein-protein interactions to be investigated in a microplate based environment. Full spectrum analysis quickly captures the entire shift in the absorbance spectrum that is seen upon protein binding to the Gold Nano Rods. Algorithms in BMG LABTECH's MARS Data Analysis software extract the necessary data from the spectra and calculate binding constants (KD) as well association (ka) and dissociation rates (kd).
For more information see the following application notes:
Label-Free SoPRano™ Gold Nano-Rod (GNR) Assays on a Spectrometer-Based Microplate Reader
Label-Free Kinetic Measurements Using SoPRano™ Gold Nano Rods (GNRs) and a Spectrometer Microplate Reader
For BMG LABTECH application notes that can be performed with the SPECTROstar Nano microplate and cuvette reader, please click here.
|Detection Modes||UV/Vis Absorbance Spectra|
|Measurement Modes ||Endpoint and Kinetic measurements|
Spectral Scanning (Absorbance)
|Microplate Formats||6 to 1536-well plates, user-definable|
LVis Plate with 16 low volume microspots (2 μL)
|Microplate Carrier||Robot compatible|
|Cuvette Port||Cuvette port for cuvettes with 10 mm path length, Micro cuvettes, Traycell compatible|
Beam height 8.5 mm
|Light Sources||High energy xenon flashlamp|
|Wavelength Selection|| UV/Vis Absorbance Spectrometer|
Full spectra or 8 distinct wavelengths in < 1 sec / well
220 - 1000 nm for ABS
|Sensitivity*||ABS with Spectrometer|
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
|Read Times||Full spectrum from 220 to 1000 nm in less than 1 sec/well|
|Shaking||Linear, orbital, and double-orbital with user-definable time and speed|
|Purge Gas Vent||System to inject an atmosphere or to pull a vacuum into the reader|
|Integrated Barcode Reader||External manual barcode reader|
|Incubation||+3°C above ambient up to 45°C|
|Software||Multi-user Reader Control and
MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant|
|Dimensions||Width: 36 cm, depth: 50, height: 16; weight: 10 kg|
|LVis Plate||Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples. |
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates.
|THERMOstar||Microplate Incubator and Shaker|
|Upgrades||Upgrades to include options such as gas vent, 1536-well format option, etc. are available. Please contact your local representative for more information.|
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
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