The PHERAstar FS is the ultimate multidetection microplate reader for HTS and assay development, especially where speed and sensitivity are imperative. The compact and versatile PHERAstar FS microplate reader is able to perform all leading non-isotopic detection technologies. Important features include: - Tandem Technology - Spectrometer / filter based detection
- Ultra fast UV/Vis spectrometer for full absorbance spectra
- Fluorescence intensity, TRF, FP, Luminescence
- Dedicated UV-laser for TR-FRET measurements
- Advanced HTRF® performance
- AlphaScreen® / AlphaLISA® laser
- On-board reagent injection for precise kinetic measurements
- Advanced top and bottom reading
- High-resolution cell layer scanning
- MARS Data Analysis Software
Due to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations, such as for FRET and BRET, the PHERAstar FS performs all assays with ease and speed. BMG LABTECH offers assay optimized and barcoded optic modules for your applications with appropriate filters, mirrors, beamsplitters, dichroics or polarizers incorporated into one hand-held module. Once inserted, the PHERAstar FS automatically selects the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader. No detection mode is compromised to accommodate the multifunctionality of the PHERAstar FS multidetection microplate reader. The PHERAstar FS can read 6-3456 well plates with uncompromised sensitivity, - no need for special apertures or optics. Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Automatic Z-height, gain adjustment, and plate mapping as well as the ability to export data in ASCII format complete the package. For HTS automation purposes, the PHERAstar FS is easily integrated into numerous robotic platforms or it can be equipped with BMG LABTECH's 50 plate Stacker. The PHERAstar FS comes with a new version of BMG LABTECH's well established Reader Control and comprehensive MARS Data Analysis Software with significant ease-of-use enhancements such as S/N, Z' and many other standard calculations. The Wizard creates an easy step-by-step calculation of a standard curve and the Template Manager is an excellent tool for building complex data processing protocols. Thus, user-defined data analysis is done simply with one click of the mouse. Improved robotic integration capabilities, digital signature and the 21 CFR part 11 compliance of MARS complete this software package.
PHERAstar FS applications include:- Protein-protein interactions (3.233)
- Affinity measurements (AN180)
- Genotyping (AN160) and reporter gene assays
- Molecular binding assays (2.1433)
- Receptor ligand studies (3.219)
- Protein phosphorylation using AlphaScreen® technology
- TR-FRET assays including HTRF®, LanthaScreen®, and LANCE®
- Cell based assays (3.233)
- DNA and Protein quantification
- G-coupled protein receptors (GPCR)
The PHERAstar FS has all of the features that one would expect from the ultimate HTS microplate reader in order to
support nearly any application that you can imagine. For a list of BMG LABTECH application notes that can be performed on the PHERAstar FS microplate reader, please click
here. AlphaScreen, AlphaLisa, Surefire, DELFIA, and LANCE are registered trademarks of PerkinElmer, Inc.
HTRF is a registered trademark of Cisbio International
LanthaScreen is a registered trademark of Invitrogen Corp.
| Detection Modes | Fluorescence Intensity - including FRET
Fluorescence Polarization / Anisotropy
High-End AlphaScreen®/AlphaLISA®
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra | | Measurement Modes | Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Real-time ratiometric measurements
Well Scanning | | Certified for | HTRF®
LanthaScreen® Certified Plus
Transcreener® far red FP
Transcreener® red FI
Transcreener® red TR-FRET | | Microplate Formats | Up to 3456-well plates, user-definable | | Microplate Carrier | Robot compatible | | Light Sources | High energy xenon flash lamp
Dedicated Laser for AlphaScreen® / AlphaLISA®
Nitrogen Laser for TRF and TR-FRET | | Detectors | Four photomultiplier tubes, optimized for different detection modes
CCD spectrometer | | Wavelength Selection | Optic Modules: Up to six applicaiton specific modules (includes all filters, dichroics, etc. for an application)
One module for top and bottom reading | | Optic Modules Capacity | Up to six application specific Optic Modules built in | | Optical Path Guides | Top and Bottom: Enclosed, free-air optical light path guided by motor-driven mirrors and dichroics | | Z-Adjustment | Automatic focal height adjustment (0.1 mm resolution) | | Spectral Range | 230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF
220 - 1000 nm for ABS | | Sensitivity* | FI (top)
< 0.4 pM (< 8 amol/well Fluorescein, 384sv, 20 μL)
< 2.0 pM (< 6 amol/well Fluorescein, 1536, 3 μL)
FI (bottom)
< 1.0 pM (< 50 amol/well Fluorescein, 384g, 50 μL)
FP
< 1.0 mP SD at 1 nM Fluorescein, 384sv, 20 μL
< 3.0 mP SD at 1 nM Fluorescein, 1536, 3 μL
TRF
< 25 fM (< 0.5 amol/well Europium, 384sv, 20 μL)
< 100 fM (< 0.8 amol/well, Europium, 1536, 3 μL)
HTRF®
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)
LUM
< 0.5 pM (< 10 amol / well ATP (384sv, 20 μL)
AlphaScreen®
< 6 pM (< 100 amol/well P-Tyr-100, 384sv, 17 μL)
ABS with Spectrometer
Spectral range: 220 - 1000 nm
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD | | Read Times | 1 flash
14 s (384), 27 s (1536)
10 flashes
38 s (384), 1 min 52 s (1536)
50 flashes
1 min 29 s (384), 5 min 18 s (1536) | | Reagent Injection | Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing | | Shaking | Linear, orbital, and double-orbital with user-definable time and speed | | Integrated Barcode Reader | Up to three integrated barcode readers | | Incubation | +5°C above ambient up to 45°C | | Software | Multi-user Reader Control and
MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant | | Dimensions | Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg | | Accessories | Optional Accessories | | Stacker | Magazines for up to 50 plates - continuous loading feature | | THERMOstar | Microplate Incubator and Shaker | | Optic Modules | Available for all applications | | Upgrades | Upgrades to include options such as additional detection modes, reagent injectors, etc. are available. Please contact your local representative for more information. | | * | LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplatets: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates |
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
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PHERAstar FS
