LUMIstar Omega - Dedicated high performance microplate luminometer upgradeable to powerful multidetection reader with spectrometer

		Luminescence including BRET

The dedicated DLReady^® certified microplate luminometer LUMIstar Omega provides highly sensitive reading of flash and glow luminescence assays and versatility through onboard "smart" injectors, a built-in filter wheel, shaking, incubation up to 60°C, as well as a gas vent connection to support all major types of cell based assays. For BRET applications, even Simultaneous Dual Emission detection is possible. Part of the Omega microplate reader series, the LUMIstar Omega also has these outstanding features:

Features:

• Onboard "smart" injectors dispense reagents and initiate kinetic events


• Upgradeable to FLUOstar Omega or POLARstar Omega with UV/Vis spectrometer


• Template manager for transferring standards, building complex data processing protocols and using default templates


• Versatile kinetic software features for endpoint, long-term and fast kinetic measurements


• Real-time kinetic monitoring


• Built-in filter wheel for color discrimination assays


• Plate capacity up to 384-well


• "QuickStart" mode allows users to read plates with just 3 mouse clicks


• Impressive well-scanning feature allows cell-based assays to be performed with ease


• Expanded temperature control up to 65°C


• Unique gas control capabilities using the microprocessor controlled ACU or purge gas vent


• Stacker and robot compatibility

Atmospheric Control Unit (ACU) - the walk away solution for cell based assays
In combination with the temperature control and unrivalled shaking options, the microprocessor controlled ACU provides a true ‘walk away’ solution for long term cell based assays. The Atmospheric Control Unit, offers fully regulated independent control of both O₂ and CO₂ levels within the LUMIstar Omega microplate luminometer. Using the ACU, O₂ levels can be programmed into the system between 1 - 19%, and CO₂ can be accurately controlled between 0 - 20%. The gases are independently regulated, thus any combination can be programmed into the unit without changing the gas cylinders or piped gas supplies.

Onboard injectors and versatile kinetic software features:
Kinetic events can be conveniently initiated using integrated "smart" injectors, which allow simultaneous reagent injection and detection. The compact and open design of the LUMIstar Omega microplate luminometer enables the user to save expensive reagents due to low dead volume and to check the priming process. Injection speed and timing is fully adjustable within the control software. Up to four different volumes, between 5 and 350 µL can be delivered in 0.5 µL increments to each well. The injectors can deliver agonists, substrates, enzymes - even cells, for advanced microplate assays. When monitoring a fast calcium signal kinetic, data can be collected as quickly as 50 reading points per second. For glow reactions, you can choose plate-mode and to have longer kinetic times or you can choose endpoint glow luminescence runs.

Color discrimination assays
The LUMIstar Omega luminometer can read at distinct wavelengths (needed for e.g. BRET and ChromaGlo") using up to eight different optical luminescence filters. These filters are built into a filter wheel, which minimizes filter switching time.

Upgradable to multidetection microplate reader with spectrometer
Whenever the need for fluorescence and absorbance based experiments arrive, have the LUMIstar Omega microplate luminometer upgraded to a FLUOstar Omega multidetection microplate reader. If the need for Simultaneous Dual Emission or Fluorescence Polarization arises, have the LUMIstar Omega upgraded to the POLARstar Omega. Both of these Omega multi-detection microplate readers are capable of capturing an absorbance spectrum (220 - 1000 nm) at a resolution of 1 nm. Read times faster than 1 second per well are possible.
For more information on the Omega series, click here.

LUMIstar Omega applications include:

• Luciferase reporter gene assays, promoter analyses (AN165, 4.778)


• ELISA immunoassays based on luminescence (4.699)


• Cell-based assays (e.g. apoptosis and viability, 4.696)


• Intracellular Ca2+ detection with aequorin


• ATP assays (e.g. Mycoplasma contaminations of cell cultures)


• GUS assays (ß-glucuronidase)


• ROS (reactive oxygen species) determination


• BRET assay for monitoring intermolecular interactions

Additional applications possible with the LUMIstar Omega benefit greatly from the microprocessor controlled ACU. Some of these applications include:

• Mammalian Cell Assays


• Hypoxia Research


• Genetic reporter assays in living cells


• Quorum sensing measured via bioluminescence


• ATP measurements to determine cell viability

The LUMIstar Omega is a dedicated luminescence detection plate reader and is DLReady certified by Promega. Onboard reagent injectors, variable shaking and top and bottom reading enable the reader to successful measure any Flash and Glow luminescence assays.

For a list of BMG LABTECH application notes that can be performed on the LUMIstar Omega microplate luminometer, please click here.

DLReady is a trademark of Promega Corp.

Detection Modes	Luminescence (flash and glow) - including BRET
Measurement Modes	Top and bottom reading Endpoint and Kinetic measurements Sequential Multi Emission measurements Simultaneous Dual Emission measurements Ratiometric measurements
Certified for	DLReady™
Microplate Formats	6 to 384-well plates, user-definable
Microplate Carrier	Robot compatible
Detectors	Low noise photomultiplier tube
Wavelength Selection	Optical Filters: Em wheels for 8 filters
Optical Filters	Emission filter wheel for 8 filters
Optical Path Guides	Top: Liquid filled light guides Bottom: Fiber optics
Spectral Range	240 - 740 nm for LUM
Sensitivity*	LUM < 1 pM (< 20 amol/well ATP, 384sv, 20 μL)
Read Times	Flying mode 20 s (96), 55 s (384)
Reagent Injection	Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 350 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing
Shaking	Linear, orbital, and double-orbital with user-definable time and speed
Purge Gas Vent	System to inject an atmosphere or to pull a vacuum into the reader
Incubation	+5°C above ambient up to 45°C or 65°C
Software	Multi-user Reader Control and MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant
Dimensions	Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
Accessories	Optional Accessories
Atmospheric Control Unit (ACU)	Oxygen Regulation: 1 - 19% using nitrogen to purge. Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge. Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg
Stacker	Magazines for up to 50 plates - continuous loading feature
THERMOstar	Microplate Incubator and Shaker
Optical Filters	Optimized for dyes, fluorophores and specific assays Filters for all applications from UV to NIR Customized filters available upon request
Upgrades	Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information.
*	LOD = 3 x SD (20 blanks) / slope (6 pt std curve) Microplatets: white for LUM 96 = 96-well microplates 384sv = 384-well small volume microplates 384g = 384-well glass bottom microplates

Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.

Additional Accessories:
Videos:
			Check out our Video Library for more videos.
Downloads:
Additional Links: Applications Compatibility BMG LABTECH Software

Click on the readers below to choose the instruments that you would like to compare to the LUMIstar Omega. Then press "Go" to start the comparison.
Please note that the order in which you click the products will be the order in which the products are shown.