The Best Drug Discovery Product 2010, FLUOstar Omega, is a multi-mode microplate reader featuring BMG LABTECH's unique Tandem Technology. It utilizes high speed UV/Vis spectrometer based absorbance and ultra sensitive filter-based detection in all detection modes such as: - UV/Vis Absorbance - measurement of complete spectra or individual wavelengths
- Fluorescence Intensity - including FRET
- Time-Resolved Fluorescence - including DELFIA®
- TR-FRET - including HTRF® and LanthaScreenTM
- AlphaScreen® / AlphaLISA®
- High-Performance Luminescence (flash and glow) - including BRET
Advanced Assay Technology Package for the FLUOstar Omega:BMG LABTECH engineers have designed a specialized measurement mode and optical system to provide the Omega multimode microplate readers with AlphaScreen ® / AlphaLISA ® performance that rivals any HTS microplate reader. For the first time, experience fantastic AlphaScreen ® / AlphaLISA ® performance on a mid-range multi-mode microplate reader. Furthermore, Advanced Performance TRF is part of the Omega's optional Advanced Assay Technology Package. It utilizes an optional high-end TRF optical reading head that incorporates an assay specific dichroic mirror. Combined with the high intensity xenon flash lamp, assay optimized filters and adjustable gain, the Omega high-end TRF reading head allows the Omega to outperform any microplate reader in the mid-range market. Additional Features:The FLUOstar Omega is based on the successful FLUOstar Optima multi-mode microplate reader and has adopted and enhanced mature hardware technology and outstanding software features: - With the LVis Plate, the FLUOstar Omega microplate reader is also capable of measuring low volume samples in a simple absorbance assay like DNA, RNA and protein concentration. The LVis Plate can measure 16 low volume samples such as DNA, RNA, or Protein. In addition, the built-in cuvette holder allows for larger sample measurements, while the optional quality control optical density (OD) and holmium oxide filters are fantastic for verifying instrument performance testing
- Unique gas control functions via the microprocessor controlled Atmospheric Control Unit (ACU), which allows for fully regulated individual control of O2 (1% to 19%) and CO2 (0% to 20%) within the microplate reader. Alternatively, BMG LABTECH offers an additional purge gas vent to replace the internal atmosphere of the microplate reader chamber with an inert gas (e.g. CO2 or Ne).
- Top and bottom reading
- Onboard "smart" injectors dispense reagents and initiate kinetic events (e.g. monitoring protein denaturation or the formation of different products in an enzymatic assay over time)
- Temperature control up to 65°C
- Microplate capacity up to 1536-well format
- Impressive well-scanning feature allows cell-based assays to be performed with ease
- Automatic optical head recognition and optics switching
- Stacker and robot compatibility
- Intuitive test protocol set-up and comprehensive data analysis with MARS software
- Template manager for transferring standards, building complex data processing protocols and using default templates
- Versatile kinetic software features for endpoint, long-term and fast kinetic measurements
- Real-time kinetic monitoring
- "QuickStart" mode allows users to read plates with just 3 mouse clicks
For more information on the Omega microplate reader series, click here.
FLUOstar Omega applications include: - Monitoring absorbance changes in cellular co-factors NADH and NADPH allowing the determination of enzymatic activity (AN170)
- ELISA, DNA and Bradford protein quantitation assays (AN169)
- Reaction optimization (metallation of porphyrin in twelve different solvents) by parallel kinetic studies (AN178)
- Aggregation assays with e.g. Thioflavin T (AN212, 2.995)
- Evaluation of PARP inhibitors (AN171)
- Measuring mitochondrial Membrane potential (AN175)
- Reporter gene assays: GFP, YFP, luciferase, dual luciferase, BRET (AN165)
- ORAC, Determination of antioxidant activity (AN197), oxygen consumption (AN207) and ROS determination (2.1002)
- Protein, DNA and RNA quantification (e.g. Bradford, Absorbance 260/280, Quant-iT PicoGreen Assay) (AN158, AN168, 2.994)
Additionally, a wide variety of applications can benefit from the Atmospheric Control Unit (ACU) for O2 / CO2 in conjunction with the FLUOstar Omega multi-mode microplate reader, including: The FLUOstar Omega is a modular multi-mode microplate reader platform for flexible assay development and cellular
analysis that allows versatility in choice of assay read out and detection mode. For a list of Application Notes that can be performed on the FLUOstar Omega multidetection microplate reader, please
click target="_blank">here. Quant-iT and PicoGreen are trademarks of Invitrogen Corp.
| Detection Modes | Fluorescence Intensity - including FRET
AlphaScreen®/AlphaLISA®
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including TR-FRET
UV/Vis Absorbance Spectra | | Measurement Modes | Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Ratiometric measurements
Well Scanning | | Certified for | HTRF®
LanthaScreen® Certified Plus
Transcreener® red FI
DLReady™
| | Microplate Formats | 6 to 1536-well plates, user-definable | | Microplate Carrier | Robot compatible | | Light Sources | High energy xenon flash lamp | | Detectors | Low noise photomultiplier tube
CCD spectrometer | | Wavelength Selection | Optical Filters: Ex and Em wheels for 8 filters each | | Optical Filters | Excitation and emission filter wheels for 8 filters each | | Optical Path Guides | Top: Liquid filled light guides
Bottom: Fiber optics | | Spectral Range | 240 - 750 nm or 240 - 900 nm for FI, FP, FRET, TRF, TR-FRET
240 - 750 nm for LUM
220 - 1000 nm for ABS | | Sensitivity* | FI
< 10 pM (< 0.2 fmol/well Fluorescein, 384sv, 20 μL)
TRF
< 1.5 pM (< 30 amol/well Europium, 384sv, 20 μL)
High-End TRF for Omega
< 0.15 pM (< 3 amol/well Europium, 384sv, 20 μL)
LUM
< 1 pM (< 20 amol/well ATP, 384sv, 20 μL)
AlphaScreen®
< 6 pM (< 100 amol/well P-Tyr-100, 384sv, 17 μL)
ABS with Spectrometer
Spectral range: 220 - 1000 nm
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD | | Read Times | Flying mode
9 s (96), 16 s (384) | | Reagent Injection | Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing | | Shaking | Linear, orbital, and double-orbital with user-definable time and speed | | Purge Gas Vent | System to inject an atmosphere or to pull a vacuum into the reader | | Incubation | +5°C above ambient up to 45°C or 65°C | | Software | Multi-user Reader Control and
MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant | | Dimensions | Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg | | Accessories | Optional Accessories | | LVis Plate | Sample Capacity: Sixteen separate microdrop wells for 2 µL samples; One standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): Four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); One holmium oxide filter for wavelength accuracy
Dimensions: Conforms to SBS standards for microplates. | | Atmospheric Control Unit (ACU) | Oxygen Regulation: 1 - 19% using nitrogen to purge.
Carbon Dioxide Regulation: 0 - 20% using 100% moisture free carbon dioxide to purge.
Dimensions (ACU only): Width: 44 cm, height: 17 cm and depth: 19 cm; Weight: 5 kg | | Stacker | Magazines for up to 50 plates - continuous loading feature | | THERMOstar | Microplate Incubator and Shaker
| | Optical Filters | Optimized for dyes, fluorophores and specific assays
Filters for all applications from UV to NIR
Customized filters available upon request | | Upgrades | Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information. | | * | LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplatets: white for LUM, AlphaScreen®, TRF; black for FI; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates |
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
Click on the readers below to choose the instruments that you would like to compare to the FLUOstar Omega. Then press "Go" to start the comparison.
Please note that the order in which you click the products will be the order in which the products are shown.
|
