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The FLUOstar Omega multi-mode microplate reader features BMG LABTECH's unique Tandem Technology utilizing high speed UV/Vis spectrometer based absorbance and ultra sensitive filter-based detection in all detection modes such as:
- UV/Vis Absorbance - measurement of complete spectra or individual wavelengths
- Fluorescence Intensity - including FRET
- Time-Resolved Fluorescence - including DELFIA®
- TR-FRET - including HTRF® and LanthaScreenTM
- AlphaScreen® / AlphaLISA®
- High-Performance Luminescence (flash and glow) - including BRET
Advanced Assay Technology Package for the FLUOstar Omega:
BMG LABTECH engineers have designed a specialized measurement mode and optical system to provide the Omega multimode microplate readers with AlphaScreen® / AlphaLISA® performance that rivals any HTS microplate reader. For the first time, experience fantastic AlphaScreen® / AlphaLISA® performance on a mid-range multi-mode microplate reader.
Furthermore, Advanced Performance TRF is part of the Omega's optional Advanced Assay Technology Package. It utilizes an optional high-end TRF optical reading head that incorporates an assay specific dichroic mirror. Combined with the high intensity xenon flash lamp, assay optimized filters and adjustable gain, the Omega high-end TRF reading head allows the Omega to outperform any microplate reader in the mid-range market.
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Additional Features:
The FLUOstar Omega is based on the successful FLUOstar Optima multi-mode microplate reader and has adopted and enhanced mature hardware technology and outstanding software features:
• Top and bottom reading
• Onboard "smart" injectors dispense reagents and initiate kinetic events (e.g. monitoring protein denaturation or the formation of different products in an enzymatic assay over time)
• Temperature control up to 60°C
• Microplate capacity up to 1536-well format
• Impressive well-scanning feature allows cell-based assays to be performed with ease
• Automatic optical head recognition and optics switching
• Stacker and robot compatibility
• Intuitive test protocol set-up and comprehensive data analysis with MARS software
• Template manager for transferring standards, building complex data processing protocols and using default templates
• Versatile kinetic software features for endpoint, long-term and fast kinetic measurements
• Real-time kinetic monitoring
• "QuickStart" mode allows users to read plates with just 3 mouse clicks
Featured Applications:
The speed of the spectrometer and the easy work-up in the software provide users with unmatched flexibility that can be used to optimize, e.g. absorbance settings for all experiments.
• Monitoring absorbance changes in cellular co-factors NADH and NADPH allowing the determination of enzymatic activity
• ELISA, DNA and Bradford protein quantitation assays
• Reaction optimization (metallation of porphyrin in twelve different solvents) by parallel kinetic studies
The FLUOstar Omega is a modular multi-mode microplate reader platform for flexible assay development and cellular analysis that allows versatility in choice of assay read out and detection mode:
• Evaluation of PARP inhibitors
• Measuring mitochondrial Membrane potential
• Ca2+ measurements (e.g. Ca2+ Flux with Fura-2, Indo-1, or FLUO-3/4)
• Enzyme activity assays (caspase, protease)
• Biomolecular interaction assays (FRET / BRET assays)
• DELFIA® assays
• Immunoassays, ELISA
• Reporter gene assays: GFP, YFP, luciferase, dual luciferase, BRET
• ORAC and ABEL antioxidant assays
• Protein, DNA and RNA quantification (e.g. PicoGreen®, NanoOrangeTM, Bradford, Lowry, Absorbance 260/280)
Click here for a list of Application Notes that can be performed on the FLUOstar Omega Multi-mode Microplate Reader.
The FLUOstar Omega multi-mode microplate reader is certified by Promega for the Dual Luciferase Reporter (DLReadyTM) gene assay, certified by Cisbio Bioassays for their HTRF® assay platform, and has LanthaScreenTM certified plus status.
For more information on the Omega microplate reader series, click here.
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HTRF is a registered trademark of Cisbio International
DLReady is a trademark of Promega Corp
DELFIA, AlphaScreen, and AlphaLISA are registered trademarks of PerkinElmer, Inc.
LanthaScreen is a trademark of Invitrogen Corp
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