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The POLARstar Omega is built on the FLUOstar Omega microplate reader platform offering all the features of the FLUOstar Omega multidetection microplate reader including BMG LABTECH's unique Tandem Technology plus fluorescence polarization and Simultaneous Dual Emission detection mode.
POLARstar Omega, the multidetection fluorescence polarization microplate reader for life science laboratories, offers the following detection modes:
• UV/Vis Absorbance - measurement of complete spectra or individual wavelengths
• Fluorescence Polarization (FP) - including Transcreener® ADP2 (96-well format)
• Fluorescence Intensity - including FRET
• Time-Resolved Fluorescence (TRF) - including DELFIA®
• TR-FRET - including HTRF® and LanthaScreen™
• AlphaScreen® / AlphaLISA®
• High-Performance Luminescence (flash and glow) - including BRET
BMG LABTECH's Dual Emission Detection System utilizes two matched PMTs allowing the detection of two emitted wavelengths at the same time. The microplate has to be read only once and any signal variations due to differences in well volumes and concentrations or fluctuations in excitation energy are corrected. Simultaneous Dual Emission Detection is especially useful for FRET, TR-FRET, BRET and Fluorescence Polarization assays. When compared to other microplate readers, the researchers benefit from this technology getting increased throughput, improved sensitivity and lower %CVs - no other reader in this price range offers simultaneous dual emission.
Advanced Assay Technology Package for the POLARstar Omega:
BMG LABTECH engineers have designed a specialized measurement mode and optical system to provide the POLARstar Omega microplate reader with AlphaScreen® / AlphaLISA® performance that rivals any HTS reader. For the first time, experience fantastic AlphaScreen® / AlphaLISA® performance on a mid-range microplate reader.
Furthermore, Advanced Performance high-end TRF is part of the Omega's optional Advanced Assay Technology Package. It utilizes an high-end TRF optical reading head that incorporates an assay specific dichroic mirror. Combined with the high intensity xenon flash lamp, assay optimized filters and adjustable gain, the high-end TRF reading head allows the POLARstar Omega to outperform any microplate reader in the mid-range market.
The POLARstar Omega is certified by Promega for the Dual Luciferase Reporter (DLR™) gene assay, certified by CisBio Bioassays for their HTRF® assay platform, and has LanthaScreen™ certified plus status. It is also validated from Bellbrook Labs for Transcreener® in 96-well plate format.
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Featured Applications
The POLARstar Omega fluorescence polarization microplate reader supports all the applications of the FLUOstar Omega. Furthermore, this fluorescence polatization microplate reader offers a fantastic speed and accuracy advantage in FRET, BRET, and FP assays used in:
• Molecular binding detection
• Receptor studies (e.g. GPCR)
• cAMP detection using FP
• Protein/protein interaction (e.g. BRET)
• Kinase profiling using a competitive FP immunoassay
• SNP Genotyping using FP
• Caspase assays using FRET-based biosensor protein
For a list of BMG LABTECH application notes that can be performed on the POLARstar Omega fluorescence polarization microplate reader, please click here.
For more information on the Omega microplate reader series, click here.
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HTRF is a registered trademark of Cisbio International
DLReady is a trademark of Promega Corp
DELFIA, AlphaLISA, and AlphaScreen are registered trademarks of PerkinElmer, Inc.
LanthaScreen is a trademark of Invitrogen Corp
Transcreener is a patented technology of Bellbrook Labs.
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