The POLARstar OPTIMA multidetection microplate reader offers all the features of the FLUOstar OPTIMA plus fluorescence polarisation and a unique simultaneous dual emission detection system, which allows the detection of two emitted wavelengths at the same time. Simultaneous Dual Emission Detection is especially useful for FRET, BRET and Fluorescence Polarisation assays since the plate only has to be read once, and when compared to other microplate readers, gives increased throughput, improved sensitivity and lower %CVs - no other reader in this price range offers simultaneous dual emission. - Fluorescence Polarisation
- Fluorescence Intensity - including FRET and multilable applications
- Time-Resolved Fluorescence - including DELFIA®
- Absorbance - UV/Vis with path length correction
- High-Performance Luminescence (flash and glow) - including BRET, DLReadyTMcertified
Additional Features:- Top and bottom reading
- Onboard "smart" injectors dispense reagents and initiate kinetic events (e.g. monitoring protein denaturation or the formation of different products in an enzymatic assay over time)
- Temperature control up to 60°C
- Impressive well-scanning feature allows cell-based assays to be performed with ease
- Automatic optical head recognition and optics switching
- Stacker and robot compatibility
- Intuitive test protocol set-up and comprehensive data analysis with MARS software
- Template manager for transferring standards, building complex data processing protocols and using default templates
- Versatile kinetic software features for endpoint, long-term and fast kinetic measurements
- Real-time kinetic monitoring
- "QuickStart" mode allows users to read plates with just 3 mouse clicks
POLARstar OPTIMA applications include: - Biomolecular interaction assays using FRET (8.113) or BRET (AN120, 9.119, 9.105, 9.103) or Fluorescence Polarization (1.133, 1.126)
- Ca2+ measurements (6.255)
- Enzyme activity and kinetics (2.952)
- DELFIA® assays (3.153)
- Immunoassays, ELISA (6.455, 6.423)
- Reporter gene assays: GFP, YFP, luciferase and DLR (4.793, 4.381)
- ORAC and ABEL antioxidant assays (AN 148)
- Protein, DNA and RNA quantification, e.g. PicoGreen®, Bradford, Lowry, Absorbance 260/280 (AN103, AN176)
- Migration assays (2.926)
This filter-based microplate reader offers a fantastic speed and accuracy advantage in FRET, BRET, and FP assays.
For BMG LABTECH application notes that can be performed on the POLARstar OPTIMA multidetection microplate reader, please click here. DLR is a registered trademark of Promega Corp.
DELFIA is a registered trademark of Perkin Elmer Inc.
PicoGreen is a registered trademark of Molecular Probes.
| Detection Modes | Fluorescence Intensity - including FRET
Fluorescence Polarization / Anisotropy
Luminescence (flash and glow) - including BRET
Time-Resolved Fluorescence - including DELFIA®
UV/Vis absorbance | | Measurement Modes | Top and bottom reading
Endpoint and Kinetic measurements
Sequential Multi Excitation measurements
Sequential Multi Emission measurements
Simultaneous Dual Emission measurements
Ratiometric measurements
Well Scanning | | Certified for | DLReady™ | | Microplate Formats | 6 to 384-well plates, user-definable | | Microplate Carrier | Robot compatible | | Light Sources | High energy xenon flash lamp | | Detectors | Two low noise photomultiplier tubes | | Wavelength Selection | Optical Filters: Ex and Em wheels for 8 filters each | | Optical Filters | Excitation and emission filter wheels for 8 filters each | | Optical Path Guides | Top: Liquid filled light guides
Bottom: Fiber optics | | Spectral Range | 240 - 750 nm or 240 - 900 nm for ABS, FI, FP, FRET, TRF, TR-FRET
240 - 750 nm for LUM | | Sensitivity* | FI
< 50pM (< 1 fmol/well Fluorescein, 384sv, 20 μL)
FP
< 5 mP SD at 1 nM Fluorescein, 96, 200 μL
TRF
< 3.5 pM (< 70 amol/well Europium, 384sv, 20 μL)
LUM
< 1.5 pM (< 30 amol/well ATP, 384sv, 20 μL)
ABS
Dynamic range: ±0.000 - 4.000 OD
Reproducibility: ±0.010 OD for 0 - 2 OD range | | Read Times | Flying mode
15 s (96), 30 s (384) | | Reagent Injection | Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 350 µL)
Variable injection speed up to 420 µL / s
Up to four injection events per well
Reagent back flushing | | Shaking | Linear, orbital, and double-orbital with user-definable time and speed | | Purge Gas Vent | System to inject an atmosphere or to pull a vacuum into the reader | | Incubation | +5°C above ambient up to 45°C or 60°C | | Software | Multi-user Reader Control and
MARS Data Analysis Software included, FDA 21 CFR Part 11 compliant | | Dimensions | Width: 44 cm, depth: 48 cm, height: 26 cm; weight: 26 kg | | Accessories | Optional Accessories | | Stacker | Magazines for up to 50 plates - continuous loading feature | | THERMOstar | Microplate Incubator and Shaker
| | Optical Filters | Optimized for dyes, fluorophores and specific assays
Filters for all applications from UV to NIR
Customized filters available upon request | | Upgrades | Upgrades to include options such as additional detection modes, reagent injectors, extended temperature control, etc. are available. Please contact your local representative for more information. | | * | LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
Microplatets: white for LUM, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates |
Not all of the features mentioned above are available in the basic units or in all detection modes. Optional accessories may be necessary to perform certain assays. Please contact your local distributor for details.
Click on the readers below to choose the instruments that you would like to compare to the POLARstar OPTIMA. Then press "Go" to start the comparison.
Please note that the order in which you click the products will be the order in which the products are shown.
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