What is the difference between endpoint mode, plate mode, and well mode readings?
February 13, 2012
- Endpoint mode experiments measure each well of the microplate only once. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization (Bellbrooks labs), et al.
- Well mode or fast kinetic experiments measure one well of the microplate multiple times (up to 50 per second) and then move onto the next well. This is usually accompanied by an injection to initiation the reaction before measurement begins. Example assays include calcium flux measurements, enzyme kinetics, ion channel activity such as VSP™ (Life Technologies), transcription assays, DLR™ (Promega), et al.
- Plate mode or slow kinetic experiments measure each well of the microplate (like endpoint), then it repeats. Example assays include bacteria and yeast growth, cell migration and invasion, enzyme kinetics, ORAC, et al.