
PHERAstar FSX
Powerful and most sensitive HTS plate reader
When studying biological reactions using microplate readers, a signal is needed for detection to determine what is going on in each well.
Microplate readers cannot just “see” DNA, proteins, or cells and usually a fluorescent or luminescent tag is necessary to illuminate the biological activity. Nature however has provided researchers with such a protein tag, that is the amino acid tryptophan. Under certain conditions tryptophan can fluorescence and be quantified. The Application Notes listed below show how several different laboratories have taken advantage of this fluorescent property of tryptophan to study the activation of heterotrimeric G proteins as well as the binding of antibodies.
Powerful and most sensitive HTS plate reader
Overwhelmed with the abundance of assay options? This blog compares the various advantages and disadvantages of different approaches available for binding assays.
In this blog you will learn what, why and how DNA interacts with different biomolecules and how microplate readers can help in their investigation.
We all heard of them: BCA assay, Bradford assay, Lowry assay. But which assay is best for me to choose for protein quantification? Find some decision support in this article.
Learn the differences between 280nm absorbance, BCA, Bradford, Nano Orange, and Lowry assays for protein quantification in this blog article.
Nitration is generally understood as the introduction of a nitro group (-NO2). Read here how nitration can influence the function of proteins during post-translational modification.