Advancing Redox Research: CLARIOstar Plate Reader at King’s College London

• Company/University: King’s College London, UK
• Interviewee: Giovanni Mann
• Products used: CLARIOstar
• Method: fluorescence intensity, luminescence, kinetic assays, cell-based assays, calcium signaling, NO and ROS studies.

This video takes you on an exclusive tour of Professor Giovanni Mann’s laboratory at King’s College London. Professor Mann’s lab is pioneering research into cellular responses under physiological oxygen conditions—levels that more accurately reflect the in vivo environment compared to standard room air. Central to this workflow is the CLARIOstar plate reader, equipped with Atmospheric Control Unit (ACU) for O₂ and CO₂ control. This technology enables luminescence and fluorescence measurements in cells pre-adapted to specific oxygen levels, maintaining consistency from culture to analysis. During the tour, Giovanni demonstrates how the CLARIOstar integrates seamlessly with Baker Ruskinn workstations to maintain consistent oxygen levels from cell culture to analysis. The CLARIOstar’s ability to rapidly modulate oxygen and inject reagents during assays allows precise investigation of processes such as calcium signaling, nitric oxide production, and reactive oxygen species generation. By replicating real tissue oxygen conditions, the system supports translational research in cardiovascular and metabolic medicine, offering insights that conventional methods cannot achieve.