Assay Technologies

Many of our Microplate Readers have received certifcation from reagent companies for their outstanding performance with various assay technologies. For more information about these certifications and assay technologies as well as which microplate readers can perform these assays, please click on the certifications below:

   

In addition we have also gathered information on the following assay technologies:

technology

Transcreener® ADP2 FP Assay

 

The Transcreener® ADP2 FP Assay from BellBrook Labs is an extremely sensitivite ADP Assay with a more sensitive antibody against ADP yielding an excellent signal at less than or equal to 10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 μM). The result is the ability to screen low ATP Km enzymes, and to use initial velocity enzyme kinetics at or below ATP Km concentrations, which leads to accurate inhibitor potencies and the ability to use less enzyme and substrate. Ratiometric, red-shifted fluorescence polarization output minimizes signal variability and reduces compound interference.

The Transcreener® ADP2 Assay is a fluorescence polarization immunoassay based on the detection of ADP by an antibody. This assay platform provides the possibility to universally interrogate all enzymes that catalyze group transfer reactions with ATP. In step one of the assay, enzymes catalyze the transfer of phosphate from ATP to a protein, peptide, lipid or small molecule resulting in the accumulation of ADP.

In step two the Transcreener® ADP2Detection Mixture, which contains an ADP Alexa633 tracer bound to an anti-ADP antibody, is added. If there is enzymatic activity resulting in necessary ADP then the bound tracer is displaced by the ADP. The free tracer rotates quickly leading to a lower polarization value. If there is no free ADP because of no enzymatic activity, the tracer is still bound to the antibody. This whole construct rotates very slowly giving a higher polarization number. Therefore, ADP production leads to a decrease in fluorescence polarization.

BMG LABTECH has also published Transcreener® ADP FP Application Notes (AN) with BellBrook Labs:

New Transcreener® ADP2 FP Assay performed on BMG LABTECH´s POLARstar Omega Microplate Reader (AN183)

New Transcreener® ADP2 FP Assay performed on BMG LABTECH´s PHERAstar Plus HTS Microplate Reader (AN 182)

Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar (AN 152)


For more information about the Transcreener® ADP2 FP Assay and other Transcreener® technologies visit:
http://www.bellbrooklabs.com

 

Transcreener is a patented technology of BellBrook Labs.