Molecular biology

Our microplate readers answer your questions on nucleic acids: concentration, interaction with other nucleic acids or proteins, single nucleotide polymorphisms and expression of genes.

Molecular biology deals with biomolecules, their structure, function, and interactions. Since the discovery and description of DNA in the 1950s, we came to understand how DNA gets first transcribed into RNA and then translated into proteins. We further understand that the genome is not the only basis for a cell’s behavior, differentiation, and pathology. Epigenetic regulation, transcription regulation and non-coding RNAs are just as important.


Today, microplate reader assays are well-established tools to answer the questions that are still open. Luminescent reporter assays study gene regulation, interaction assays examine binding of nucleic acids to proteins and enzyme activity assays report on activities of polymerases or helicases.

 

Genomics is a multidisciplinary and essential part of modern biology.  Next generation sequencing (NGS) and SNP-genotyping are popular tools. The field is also closely associated with transcription and translation, investigating proteins and expression levels, both to directly inform studies but also as part of analysis workflows such as NGS.
Microplate reader also take measurements in preparation for PCRs and sequencing. They quantify nucleic acids in high throughput (96-1536 wells) and determine their purity via absorbance spectra.

 

BMG LABTECH microplate readers offer solutions from simple quantification devices able to quantify DNA, RNA and protein efficiently and in high throughput, up to multi-functional devices suitable for all needs: reporter assays, binding assays, enzyme assays, no matter which detection mode.

Resources

Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.

    • Regulation of IL-6/STAT-3/Wnt axis by nifuroxazide dampens colon ulcer in acetic acid-induced ulcerative colitis model: Novel mechanistic insight

      Ali, FEM;Elfiky, M;Fadda, WA;Ali, HS;Mahmoud, AR;Mohammedsaleh, ZM;Abd-Elhamid, TH;[2021]

      Life sciences

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    • The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer

      Golden, E;Rashwan, R;Woodward, EA;Sgro, A;Wang, E;Sorolla, A;Waryah, C;Tie, WJ;Cuyàs, E;Ratajska, M;Kardaś, I;Kozlowski, P;Johnstone, EKM;See, HB;Duffy, C;Parry, J;Lagerborg, KA;Czapiewski, P;Menendez, JA;Gorczyński, A;Wasag, B;Pfleger, KDG;Curtis, C;Lee,[2021]

      Nature communications

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    • A proof-of-concept study for the pathogenetic role of enhancer hypomethylation of MYBPHL in multiple myeloma

      Wong, KY;Morgan, GJ;Boyle, EM;Cheng, ASL;Yip, KY;Chim, CS;[2021]

      Scientific reports

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    • A vaccine targeting mutant IDH1 in newly diagnosed glioma

      Platten, M;Bunse, L;Wick, A;Bunse, T;Le Cornet, L;Harting, I;Sahm, F;Sanghvi, K;Tan, CL;Poschke, I;Green, E;Justesen, S;Behrens, GA;Breckwoldt, MO;Freitag, A;Rother, LM;Schmitt, A;Schnell, O;Hense, J;Misch, M;Krex, D;Stevanovic, S;Tabatabai, G;Steinbach, [2021]

      Nature

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    • Human MC4R variants affect endocytosis, trafficking and dimerization revealing multiple cellular mechanisms involved in weight regulation

      Brouwers, B;de Oliveira, EM;Marti-Solano, M;Monteiro, FBF;Laurin, SA;Keogh, JM;Henning, E;Bounds, R;Daly, CA;Houston, S;Ayinampudi, V;Wasiluk, N;Clarke, D;Plouffe, B;Bouvier, M;Babu, MM;Farooqi, IS;Mokrosiński, J;[2021]

      Cell reports

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