Read which methods are available to measure influences on the expression of a specific gene.
Molecular biology deals with biomolecules, their structure, function, and interactions. Since the discovery and description of DNA in the 1950s, we came to understand how DNA gets first transcribed into RNA and then translated into proteins. We further understand that the genome is not the only basis for a cell’s behavior, differentiation, and pathology. Epigenetic regulation, transcription regulation and non-coding RNAs are just as important.
Today, microplate reader assays are well-established tools to answer the questions that are still open. Luminescent reporter assays study gene regulation, interaction assays examine binding of nucleic acids to proteins and enzyme activity assays report on activities of polymerases or helicases.
In addition to answering molecular biology questions, the reader also takes measurements in preparation for PCRs and sequencing. It quantifies nucleic acids in high throughput (96-1536 wells) and determines their purity via absorbance spectra.
BMG LABTECH microplate readers offer solutions from simple quantification devices with high-throughput and automation capabilities, up to multi-functional devices suitable for all needs: reporter assays, binding assays, enzyme assays, no matter which detection mode.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
DNA quantification using absorbance (A260) and fluorescent methods (Qubit™ and Quant-iT™/PicoGreen™)Andrea Krumm, BMG LABTECH GmbH, 77799 Ortenberg, Germany, 10/2020
Qubit™ RNA Quantiﬁcation of in vitro transcribed crRNAs using an Echo liquid handler and the CLARIOstar PlusMichael Andrew Crone (1,2) , Manoja Rasamanikkam (3), (1) Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, SW7 2AZ , (2) UK Dementia Research Institute Care Research u. Technology Centre at Imperial College London , (3) BMG LABTECH, Aylesbury, UK, 04/2020
A protein-based biosensor for mRNAAlexander Cook , Christopher P. Toseland, School of Biosciences, University of Kent, UK, 12/2018
Fluorescence polarization-based RNA synthesis assayStefan Reich , Stephen Cusack, European Molecular Biology Laboratory, 10/2018
Streamlining Next Generation Sequencing quality management using high throughput fluorometric DNA/RNA quantificationUlrike Bönisch , Laura Arrigoni , Diana Santacruz , Nadia Kress, Deep Sequencing Facility, Max Planck Institute of Immunobiology and Epigenetics Freiburg, 09/2018
Next Generation Sequencing (NGS), why quantifying exact DNA/RNA is important
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