Read which methods are available to measure influences on the expression of a specific gene.
Molecular biology deals with biomolecules, their structure, function, and interactions. Since the discovery and description of DNA in the 1950s, we came to understand how DNA gets first transcribed into RNA and then translated into proteins. We further understand that the genome is not the only basis for a cell’s behavior, differentiation, and pathology. Epigenetic regulation, transcription regulation and non-coding RNAs are just as important.
Today, microplate reader assays are well-established tools to answer the questions that are still open. Luminescent reporter assays study gene regulation, interaction assays examine binding of nucleic acids to proteins and enzyme activity assays report on activities of polymerases or helicases.
Genomics is a multidisciplinary and essential part of modern biology. Next generation sequencing (NGS) and SNP-genotyping are popular tools. The field is also closely associated with transcription and translation, investigating proteins and expression levels, both to directly inform studies but also as part of analysis workflows such as NGS.
Microplate reader also take measurements in preparation for PCRs and sequencing. They quantify nucleic acids in high throughput (96-1536 wells) and determine nucleic acid purity via absorbance spectra.
BMG LABTECH microplate readers offer solutions from simple quantification devices able to quantify DNA, RNA and protein efficiently and in high throughput, up to multi-functional devices suitable for all needs: reporter assays, binding assays, enzyme assays, no matter which detection mode.
Search our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
DNA purity ratio – fast and easy absorbance-based evaluation of nucleic acid qualityAnn-Cathrin Volz, BMG LABTECH, 77799 Ortenberg, Germany, 09/2021
LAMP assay for detecting SARS-CoV-2 RNA using an absorbance-based colorimetric readoutAnn-Cathrin Volz, , Martin Mangold, BMG LABTECH 77799 Ortenberg, Germany, 03/2021
Studying the molecular mechanism of viral replication in real time using the CLARIOstar Plus with ACUMarko Noerenberg (1) , Vincenzo Ruscica (2) , Alfredo Castello (1,2*), (1) MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland (UK) , (2) Department of Biochemistry, University of Oxford, South Parks road, OX1 3QU, Oxford, (UK) *Correspondence to: firstname.lastname@example.org, 11/2020
DNA quantification using absorbance (A260) and fluorescent methods (Qubit™ and Quant-iT™/PicoGreen™)Andrea Krumm, BMG LABTECH GmbH, 77799 Ortenberg, Germany, 10/2020
Qubit™ RNA Quantiﬁcation of in vitro transcribed crRNAs using an Echo liquid handler and the CLARIOstar PlusMichael Andrew Crone (1,2) , Manoja Rasamanikkam (3), (1) Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, SW7 2AZ , (2) UK Dementia Research Institute Care Research u. Technology Centre at Imperial College London , (3) BMG LABTECH, Aylesbury, UK, 04/2020
Next Generation Sequencing (NGS), why quantifying exact DNA/RNA is important
Förster Resonance Energy Transfer Nanoplatform Based on Recognition-Induced Fusion/Fission of DNA Mixed Micelles for Nucleic Acid SensingRead article
YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effectRead article
Cell death and differentiation
Fine mapping identified the gibberellin 2-oxidase gene CpDw leading to a dwarf phenotype in squash (Cucurbita pepo L.)Read article
A systematic CRISPR screen defines mutational mechanisms underpinning signatures caused by replication errors and endogenous DNA damageRead article
Regulation of IL-6/STAT-3/Wnt axis by nifuroxazide dampens colon ulcer in acetic acid-induced ulcerative colitis model: Novel mechanistic insightRead article