
PHERAstar FSX
Powerful and most sensitive HTS plate reader
Luminescence assays come in many different types.
Basically luminescence assays are a chemical or enzymatic reaction that uses a substrate. When the substrate is acted upon by the chemical or enzymatic reaction, light is given off as a by-product. The two main types of luminescence reactions are Flash and Glow. The Flash luminescence reaction occurs quickly, in a matter of seconds or minutes, giving off a very bright signal. Whereas Glow luminescence assays can last for hours but they are not as bright as Flash luminescence assays.
An example of the Flash luminescence assay is DLR® from Promega:
An example of a Glow luminescence assays is the ADP-Glo® Kinase assay from Promega:
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HTRF combines time-resolved fluorescence detection with FRET and is mainly used for drug screening in high-throughput. Read more about this measurement approach here.
ELISAs are irreplaceable tools in the life sciences. This article explains the principle of ELISA assays and presents adjustments for optimal performance.
This article clarifies what integration time is, why it is important and how you can optimise the integration time for TR-FRET measurements.
Dual-reporter assays with stable, long-lived luminescence reporters that emit at different wavelengths offer several advantages for higher-throughput detection. Read more here.