What is the difference between Flash and Glow luminescence assays?

Luminescence assays come in many different types.

Dr EJ Dell Dr EJ Dell

Basically luminescence assays are a chemical or enzymatic reaction that uses a substrate. When the substrate is acted upon by the chemical or enzymatic reaction, light is given off as a by-product. The two main types of luminescence reactions are Flash and Glow. The Flash luminescence reaction occurs quickly, in a matter of seconds or minutes, giving off a very bright signal. Whereas Glow luminescence assays can last for hours but they are not as bright as Flash luminescence assays.

Both assay types can be measured with a luminescence microplate reader. However, for flash assays injectors are typically required because of the fast onset of the reaction.

An example of the Flash luminescence assay is DLR® from Promega:
An example of a Glow luminescence assays is the ADP-Glo® Kinase assay from Promega:




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