Most flexible Plate Reader for Assay Development
Learn how the CLARIOstar with ACU replicates in vivo oxygen conditions to increase the significance of your data
Cell-based assays are typically run at non-physiologically relevant O2 levels such as 21%. The speakers show the impact that working under physiological conditions can have to improve the interpretation of data. They present a case study employing a Cardiovascular Endothelial line in which Nrf-2 activity is investigated using ROS and cell metabolism measurements.
The scientific talk explores how and why O2 as a physiological parameter can influence data. The interpretation of disease models will be demonstrated and researchers learn how to adopt a similar strategy to more accurately replicate the in vivo condition on the bench.