Come and learn more about BMG LABTECH at these three workshops at the SBS Conference:
Workshop 1:
Investigate GPCR Functional Selectivity with Fluorescence Based Assays from Invitrogen and BMG LABTECH’s PHERAstar FS HTS Instrument
Date: Monday, April 12, 2010
Time: 1:40 - 2:15 p.m.
Room: 122AB
Robust fluorescent cell-based assays, suitable for HTS, have been developed by Invitrogen to examine GPCRs. Using BMG LABTECH’s next generation HTS microplate reader, the PHERAstar FS, GPCR signaling pathways are readily evaluated. New and unique features on the PHERAstar FS expand the compatibility of different assay formats and provide fantastic results. Calcium mobilization using the Fluo-4 Direct™ calcium assay and beta- arrestin recruitment using the Tango™ GPCR cellular assay will be highlighted.
Workshop 2:
Profiling Kinases and ATPases Using Promega's ADP-Glo(tm) Kinase Assay on BMG LABTECH's PHERAstar and FLUOstar Microplate Readers
Date: Wednesday, April 14, 2010
Time: 8:00- 8:45 a.m.
Room: 122AB
Profiling of protein kinase inhibitors for selectivity is a common practice in drug discovery. Although radioactive assays have been used traditionally for such purposes, robust, non-radioactive approaches are desired. The ADP-Glo™ Kinase Assay measures kinase activity by quantifying the amount of ADP produced during the reaction in a homogeneous, bioluminescent format. It is proving to be as sensitive as traditional radioactive approaches.
Workshop 3:
A Fast and Simple Method for Measuring P-Glycoprotein (Ppg) Inhibition - The Fluorosome®-trans-pgp Assay and the FLUOstar Omega
Date: Wednesday, April 14, 2010
Time: 1:30- 2:15 p.m.
Room: 127AB
The Fluorosome Company’s Fluorosome®-trans-pgp assay, together with BMG LABTECH’s FLUOstar Omega, provide a rapid, specific and sensitive reconstituted liposome assay platform for P-glycoprotein (Pgp) inhibition and IC50 determinations. Major advantages of this direct assay for P-glycoprotein/drug interactions are specificity and speed. The FLUOstar Omega’s ability to take fifty readings per second concurrently with injection allows for 10 point Pgp IC50 determinations in only 10 minutes.
