Assay Technologies

Many of our Microplate Readers have received certifcation from reagent companies for their outstanding performance with various assay technologies. For more information about these certifications and assay technologies as well as which microplate readers can perform these assays, please click on the certifications below:

   

In addition we have also gathered information on the following assay technologies:

technology

MycoAlert

Mycoplasma can influence experimental results obtained for a certain cell line, therefore, cell culture labs need to check regularly if their cultures are contaminated by mycoplasma species. The MycoAlert™ Mycoplasma Detection Kit from Lonza is specific for mycoplasmal enzymes that are not present in eukaryotic cells. After addition of substrate a high luminescent signal is created only in viable mycoplasma. Measurements of luminescence are taken before and after addition of MycoAlert™ substrate. From these measurements a ratio is built.

Ratio interpretation

< 0.9 negative for mycoplasma
0.9 – 1.2 borderline: quarantine cells & retest in 24 h
> 1.2 mycoplasma contamination
 

Example of a MycoAlert assay on BMG LABTECH instruments


See a complete list of luminescence application notes.

The BMG LABTECH instruments come with the MARS Data Analysis software that allows users to do calculations and validations to quickly identify samples which may be contaminated.