Many of our Microplate Readers have received certifcation from reagent companies for their outstanding performance with various assay technologies. For more information about these certifications and assay technologies as well as which microplate readers can perform these assays, please click on the certifications below:
In addition we have also gathered information on the following assay technologies:
The Dual-Luciferase® Reporter (DLR™) Assay System from Promega Corp. provides a luminescent reporter assay to study gene transcription and regulation. The activities of two luciferases are measured sequentially from a single sample reporting the transcription of interest and a transfection control.
Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors, such as in the Omega Series and PHERAstar FSX microplate readers. In the DLR™ Assay System, both reporters yield linear assays with attomole sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.
Dual Luciferase Reaction - Luciferase Assay Reagant II (LAR II) is injected in the first step and the Firefly reaction is started. Stop and Glo® buffer is injected in the second step, which quenches the Firefly reaction and initiates the Renilla reaction
BMG LABTECH has published Application Notes (AN) in cooperation with Promega that demonstrate the use of DLR™ technology with BMG LABTECH microplate readers.
Dual Luciferase Reporter (DLR™) Assay Certification on the Omega Series of Readers (AN 165)
Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon (AN 172)
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega (AN184)
For more information on DLR ratio Evaluation, take a look at the following video:
This video from BMG LABTECH shows Test Protocol Settings for an automated Dual Luciferase kinetic reaction measurement including various result calculations done with the comprehensive Data Analysis Software MARS.
For more information about DLR™, please see Promega's website: www.promega.com