Application Notes sorted by Detection Mode

Absorbance
[199 - 4.330]
Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA
Eboigbodin KE and Robinson GK
BMG LABTECH 2009
Article
[185 - 6.250]
Enzyme kinetic measurements performed on BMG LABTECH´s FLUOstar OPTIMA
Franka Ganske
BMG LABTECH 2009
Esterase activity was determined using the p-nitrophenyl acetate (pNPA) assay. Km and Vmax were calculated using different calculation methods (e.g. Michaelis-Menten and Lineweaver-Burk).
Article
[191 - 6.270]
High-Throughput Monitoring of Bacterial Growth at Elevated Hydrostatic Pressure on the FLUOstar OPTIMA
S. Lucas Black, F. Bruce Ward and Rosalind J. Allen
BMG LABTECH 2009
Photobacterium profundum SS9 is a piezophile: it grows better at elevated hydrostatic pressure than at atmospheric pressure. The presented growth rates are influenced by pressure, sealing technique and osmolarity.
Article
[189 - 6.260]
High-Throughput Method for Dual Assessment of Antifungal Activity and Growth Kinetics Using a FLUOstar Omega
Gordon Ramage, Ryan Quinn and Steven Milligan
BMG LABTECH 2009
The effects of the antifungal tea tree oil on the growth of Candida albicans were measured in a long-time kinetic.
Article
[184 - 6.240]
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega
Mutch BE, Willetts K, Timms E, Foxwell BMJ
BMG LABTECH 2008
The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR).
Article
[178 - 6.230]
Reaction optimization by parallel kinetic studies with the FLUOstar Omega
Redman JE
BMG LABTECH 2008
Relative reaction rates of the metallation of tetraphenylporphyrin (TTP) with zinc were measured. The values for twelve different solvents were determined in parallel.
Article
[170 - 6.210]
Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
Dell EJ
BMG LABTECH 2008
UV absorbance spectra of NAD+ and NADH as well as NADP+ and NADPH show why it is possible to quantify cofactor-dependent reactions by absorbance measurements at 340 nm.
Article
[171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
Rebecca Foster and Kyla Grimshaw
BMG LABTECH 2008
Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay.
Article
[169 - 6.200]
Old Assays, New Instrument: ELISA; NADH and NADPH Conversion; DNA and Protein Quantitation
Dell EJ and Ganske F
BMG LABTECH 2008
Classic lab UV absorbance assays are summarized. DNA, protein and cofactor spectra are presented.
Article
[167 - 6.180]
Comparison of Thioredoxin Activity in Cortical Neurons and Glial Cells using the FLUOstar OPTIMA
Papadia S, Léveillé F and Hardingham GE
BMG LABTECH 2008
The thioredoxin assay used in this application note is based on different redox reactions leading to the release of thionitrobenzoic acid. The resulting bright yellow absorbance signal is measured at 405 nm.
Article
[168 - 6.190]
Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
Ganske F
BMG LABTECH 2008
DNA measurements using UV absorbance at 260 nm are shown. Limit of DNA detection and special software features are presented.
Article
[158 - 6.170]
Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software
Ganske F, Dell EJ
BMG LABTECH 2007
The Bradford assay was used to quantify proteins in solution. The spectrum shift can be followed online during the measurement in the current state window.
Article
[145 - 6.140]
Plasso EpranEx" ready to use heparin-binding plate with the FLUOstar OPTIMA plate reader
Wiles M.
Plasso Technology Ltd., Sheffield, UK
Article
[140 - 6.130]
High-Throughput Determination of Bacterial Growth using a FLUOstar OPTIMA
Webber MA, Piddock LJ
BMG LABTECH 2006
The biocides Superkill, Farm Fluid S and Virkon S were used to screen for tolerant mutants of Salmonella enterica serovar Typhimurium.
Article
[135 - 2.430]
Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader
Poon S, Rivers RC
Department of Chemistry, University of Cambridge, UK.
Article
[116 - 6.002]
DNA Quantification (Absorbance Mode)
BMG LABTECH, Germany
The most common method for quantifying DNA samples is by conventional absorbance measurements.
Article
AlphaScreen®
[190 - 7.130]
Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus
Franka Ganske and Keith Ansell
BMG LABTECH 2009
Quantitative determination of the most present immunoglobulin in plasma: IgG. Standard curves and PHERAstar Plus instrument settings for AlphaLISA® are presented.
Article
[186 - 7.120]
AlphaScreen® Phosphotyrosine Assay Performed on a FLUOstar Omega and a POLARstar Omega
Franka Ganske
BMG LABTECH 2008
In this application note AlphaScreen® instrument settings and sensitivity as well as Z´value of a tyrosine kinase assay are presented. The measurements were done using Omega instruments.
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[153 - 7.110]
Detection of tyrosine kinase activity using the PHERAstar in AlphaScreen® mode
Ganske F and Orban M.
BMG LABTECH, Germany, 2007
Laser-based AlphaScreen® is demonstrated in this application note. PHERAstar instrument settings are given and a titration curve of biotinylated and phosphorylated polypeptide (biot-LCK-P) is presented.
Article
Fluorescence Intensity
[206 - 2.680]
Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader
Mark Koeppel, Invitrogen, Madison Wisconsin, USA; EJ Dell, BMG LABTECH, NC, USA
BMG LABTECH 2010
Article
[204 - 2.670]
Analysis of migration using the OrisTM Cell Migration Assay-TriCoated kit on the Polarstar Omega
Iman van den Bout
BMG LABTECH 2009
Article
[203 - 2.650]
Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
George Cathcart, Brendan Gilmore, Brian Walker.
BMG LABTECH 2009
Article
[201 - 2.660]
The Transcreener® ADP2 FI Assay performed on PHERAstar and Omega microplate readers
Franka Ganske, BMG LABTECH, Offenburg, Germany; EJ Dell, BMG LABTECH, NC, USA / Meera Kumar, BellBrook Labs, Madison, USA
BMG LABTECH 2009
Article
[197 - 2.630]
ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader
Franka Ganske und Marjan Orban
BMG LABTECH 2009
Article
[196 - 2.620]
Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation
Muller RE, Siderovski DP, Kimple AJ, Dell EJ
BMG LABTECH 2009
Article
[195 - 2.610]
alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA
Markaki AE
BMG LABTECH 2009
Article
[188 - 2.600]
A Leukocyte Adhesion Assay Performed on BMG LABTECH's FLUOstar OPTIMA
Sanders, D.A. and Charnock-Jones, D.S.
BMG LABTECH 2009
A leukocyte adhesion assay was utilized to screen for potential inflammatory markers. Primary peripheral blood mononuclear cells and different leukocyte cell lines (HL60, JY and U937) were investigated.
Article
[179 - 2.580]
Fluorometric determination of extracellular enzyme activities in peat using the FLUOstar OPTIMA
Bonnett, S.A.F., Leah, R., and Maltby, E
BMG LABTECH 2008
Article
[176 - 2.570]
Rapid ultra-sensitive isothermal DNA detection using RPA technology and the POLARstar OPTIMA
Olaf Piepenburg and Niall A. Armes
BMG LABTECH 2008
Article
[175 - 2.560]
Measuring Mitochondrial Membrane Potential using the FLUOstar Omega Microplate Reader
Heather Mortiboys and Oliver Bandmann
BMG LABTECH 2008
Article
[159 - 2.510]
High-Throughput Method for Dynamic Measurements of Cellular Viability Using a FLUOstar OPTIMA
Chitolie MS & Toescu EC
BMG LABTECH 208
Article
[171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
Rebecca Foster and Kyla Grimshaw
BMG LABTECH 2008
Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay.
Article
[166 - 2.540]
Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
Tracey Madgett, University of the West of England, Bristol
BMG LABTECH 2008
Article
[164 - 2.530]
Use of the FLUOstar OPTIMA Microplate Reader to monitor amyloid formation
Sarah Shammas and Ann-Christin Brorsson
Department of Chemistry and Cavendish Laboratory, University of Cambridge
Article
[162 - 2.520]
Investigation of the stereoselectivity of an anti-amino acid antibody utilizing tryptophan fluorescence
Danielle M. Corgliano, Heike Hofstetter and Oliver Hofstetter
Northern Illinois University, DeKalb, IL, USA
Article
[157 - 2.500]
Calcium Detection Upon GPCR Stimulation Using the NOVOstar and Screen Quest
Liao J, Dell E.J.
ABD Bioquest, Sunnyvale, CA, USA, BMG LABTECH Inc, Durham, NC, USA 2007
Article
[156 - 2.490]
Internalisation of Fluorescently Labelled Phagocytosis Targets Quantified by the FLUOstar OPTIMA
Taylor A, Donelly L.
Thoracic Medicine, National Heart and Lung Institute
Article
[146 - 2.470]
Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans
Balda MS and Matter K
BMG LABTECH 2007
Article
[148 - 2.480]
ORAC Assay on the FLUOstar Optima to Determine Antioxidant Capacity
Ganske F, Dell EJ
BMG LABTECH
Article
[144 - 2.460]
Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlok and FLUOstar OPTIMA
Hart CA , Clarke NW, Brown MD
Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
Article
[141 - 2.450]
ADP HunterTM Assay for HTS of Kinase Inhibitors using the PHERAstar
Kauffman L, Hguyen U, Angersbach S, Orban M
BMG LABTECH 2006
Article
[138 - 2.440]
Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Worzella T, Larson B
BMG LABTECH 2006
Article
[135 - 2.430]
Protein Aggregation Monitoring on the FLUOstar OPTIMA Microplate Reader
Poon S, Rivers RC
Department of Chemistry, University of Cambridge, UK.
Article
[128 - 2.410]
High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader
Aucamp J, Cosme AM, Lye GJ, Dalby PA
BMG LABTECH 2005
Article
[132 - 2.400]
Development of a post-reaction derivatisation and Fluorescence assay for 2-OG dependent dioxygenases
McNeill LA, Hewitson KS, Schonfeld CJ, Bethge L.
BMG LABTECH 2004
Article
[103 - 2.018]
Molecular Probes PicoGreen assay performed on BMG LABTECH POLARstar OPTIMA microplate reader
Angersbach S, Earp R.
BMG LABTECH
Article
[104 - 2.025]
Intracellular Calcium Assay performed on NOVOstar Microplate Reader
Heding A.
BMG LABTECH
Article
[107 - 2.013]
Rapid functional screening at dopamine receptors by measuring intracellular calcium with the NOVOstar fluorescence reader
Hoefgen B, Kassack MU.
Application Note PDF
[105 - 2.008]
Fluorescence analysis of reactive oxygen species by six isolates of Aspergillus Fumigatus
Hipler UC, Wollina U, Denning D, Hipler B.
Application Note PDF
Fluorescence Resonance Energy Transfer (FRET)
[194 - 8.150]
FRET-based screening for potential modulators of the Galphai1 protein/GoLoco interaction
Hutsell SQ, Kimple AJ, Siderovski DP
BMG LABTECH 2009
Article
[160 - 8.130]
High Speed FRET based SNP Genotyping Measurement on the PHERAstar
Robinson P and Ganske F
BMG LABTECH 2007
Article
[163 - 8.140]
SUMO FRET-based assays performed on BMG LABTECH's NOVOstar
Martin SF
Article
[129 - 8.120]
Use of the PHERAstar for SNP genotyping chemistry development and high throughput production genotyping
Robinson P.
BMG LABTECH 2004
Article
[123 - 8.110]
Ion channel assay development using Invitrogen's FRET-based voltage sensor probes
Hoffman RL, Duff S, Qadir N.
Invitrogen Corporation, Madison, WI 53719, USA
Article
[122 - 8.100]
Use of CyDye Fluors for Improved FRET Protease Assays on BMG POLARstar Fluorescence Microplate Reader
George J, Norey C.
GE Healthcare
Article
Time-Resolved Fluorescence and TR-FRET
[192 - 3.180]
A DELFIA® time-resolved fluorescence cell-mediated cytotoxicity assay performed on the PHERAstar
Patterson NM, Cunningham T, Jiang X, Dell EJ, Ganske F, Shapiro DJ
BMG LABTECH 2009
Article
[193 - 3.190]
Functional Screening of recombinant CHO-M1 cells using the PHERAstar from BMG LABTECH
Bernd Laffert and Franka Ganske
BMG LABTECH
HTRF IPOne assay with fresh cells from a growing culture and Frozen Instant Cells measured at Cell Culture Service (CCS), Hamburg, Germany.
Article
[177 - 3.170]
HTRF®IP-One Terbium-based assay performed on BMG LABTECH´s PHERAstar Plus
Ganske F, Jacquemart L, Jean A, Lebreton ML, Tardieu JL
BMG LABTECH 2008
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[154 - 3.160]
Cisbio´s HTRF® cortisol assay performed on the PHERAstar
Ganske F, Tardieu JL and Degorce F.
BMG LABTECH, Germany and Cisbio International, France
Article
[137 - 3.130]
HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
Orban M, Degorce F, Tardieu JL
BMG LABTECH 2005
Article
[127 - 3.127]
A novel PI 3-Kinase assay utilising HTRF® technology on the RUBYstar
Rossant CJ, Brown JP, Marshall ML.
BMG LABTECH 2004
Article
Fluorescence Polarization
[205 - 1.190]
Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
Kushnareva Y, Dell EJ
BMG LABTECH 2009
Article
[183 - 1.180]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's POLARstar Omega
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
Article
[182 - 1.170]
New Transcreener® ADP2 FP Assay performed on BMG LABTECH's PHERAstar Plus HTS Microplate Reader
Larson B, Ganske F, Dell EJ
BMG LABTECH 2008
Article
[180 - 1.160]
High-throughput protein-DNA affinity measurements using fluorescence anisotropy
Veprintsev DB
BMG LABTECH 2008
Article
[173 - 1.150]
Performance of the PredictorTM hERG Fluorescence Polarization Assay Kit using the PHERAstar
Dell EJ
BMG LABTECH 2008
Article
[152 - 1.140]
Transcreener® ADP Fluorescence Polarization Assay Performed on the PHERAstar
Ganske F, Dell EJ, Larson B
BMG LABTECH 2008
Article
[126 - 7.100]
PHERAstar: A Next Generation Multimode Plate Reader for Sophisticated HTS Assays
Orban M, Angersbach S, Hoffman R, Buros M.
BMG LABTECH 2005
Herein we show the capability of the PHERAstar to measure challenging assays in HTS well format. Assays include Far Red PolarScreen® Kinase, cAMP AlphaScreen® and TNFa using the HTRF® technique.
Application Note PDF
[142 - 1.030]
HitHunterTM IP3 Assay for GCPR Screening using the PHERAstar
Kauffman L, De La Llera, Angersbach S, Orban M
BMG LABTECH 2006
Article
[124 - 1.020]
Identifcation of false positives in an FP Screen
Saxty B
BMG LABTECH 2004
Article
[119 - 1.101]
Fluorescence polarization discriminates green fluorescent protein from interfering autofluorescence
Knight AW
BMG LABTECH
Article
Luminescence
[202 - 4.340]
Promega's ADP-Glo(TM) Kinase Assay performed on BMG LABTECH's PHERAstar FS and POLARstar Omega
Ganske, F, Dell, EJ, Shultz S
BMG LABTECH 2009
Article
[199 - 4.330]
Monitoring Bacterial Cell-to-Cell Communication "Quorum sensing" Using FLUOstar OPTIMA
Eboigbodin KE and Robinson GK
BMG LABTECH 2009
Article
[184 - 6.240]
Multiplex Analysis of Inflammatory Cytokines from Primary Human Macrophages using a FLUOstar Omega
Mutch BE, Willetts K, Timms E, Foxwell BMJ
BMG LABTECH 2008
The protein concentration of different inflammatory cytokines (e.g. TNFa or IL-6) were determined with the help of ELISA and Luciferase Reporter Gene Assay (DLR).
Article
[172 - 4.300]
Dual luciferase assay to assess the replication of the hepatitis C virus subgenomic replicon
Gretton S, Harris M
BMG LABTECH 2008
Article
[171 - 4.290]
Evaluation of PARP Inhibitors: Performed on BMG LABTECH's FLUOstar Omega
Rebecca Foster and Kyla Grimshaw
BMG LABTECH 2008
Poly (ADP-ribose) polymerases (PARP) play a crucial role in post-translational modifications. PARP inhibitors were determined using a chemiluminescent assay.
Article
[165 - 4.280]
Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers
Dell EJ
BMG LABTECH 2007
Article
[151 - 4.250]
Promega´s P450-Glo Luminescent Cytochrome P450 Assay Using the FLUOstar OPTIMA
Cali J, Dell EJ
Promega Corporation, BMG LABTECH, Germany
Article
[147 - 4.240]
LUMIstar in the production and quality control for ROTAS on-site test reagents
Dix A
Cybersense Biosystems Ltd, Oxon, UK
Article
[143 - 4.230]
Study of GPCR pharmacology using the DiscoveRx HitHunter
Rainard JM, Mireylees SE, Darlison MG
BMG LABTECH 2006
Article
[139 - 4.220]
Promega's Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
Worzella T, Larson B
BMG LABTECH 2006
Article
[134 - 4.200]
New HitHunter cAMP XS+ Assay for GPCR Screening Using the PHERAstar
Kauffman L, De La Llera S, Orban M
BMG LABTECH 2006
Article
[138 - 2.440]
Promega's Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Worzella T, Larson B
BMG LABTECH 2006
Article
[136 - 4.210]
LUX Biotechnology GLOWELL luminometry standards on the POLARstar OPTIMA
Perfect E, Hickey P.
BMG LABTECH 2004
Article
[130 - 4.170]
Cambrex Bio Science MycoAlert Assay on the LUMIstar plate reader
Cox A, Pitt A.
BMG LABTECH 2004
Article
[131 - 4.180]
Cambrex PKLight Protein Kinase Assay on the PHERAstar plate reader
Paine A, Walker L.
BMG LABTECH 2004
Article
[112 - 4.103]
Use of the LUMIstar Galaxy luminometer to monitor the production of free radicals by leucocytes in diluted blood
Lateef S, Ingram PR, Spickett CM.
Article
[110 - 4.012]
Promega ENLITEN Kit on the FLUOstar Galaxy and LUMIstar Galaxy
Earp RL, Cheek J
BMG LABTECH
Article
[108 - 4.009]
Luminescent ABEL® Antioxidant Test Kit with PHOLASIN® for Vitamin C type antioxidants
Hipler B, Knight J.
Article
[113 - 4.007]
The use of an ATP bioluminescence assay to quantify cell cytotoxicity
Hipler B.
BMG LABTECH
Article
[111 - 4.006]
Chemiluminescence measurement of the generation of reactive oxygen species
Hipler B, Hipler UC.
Article
[106 - 4.002]
Inhibitory effect of terbinafine on reactive oxygen species generated by candida albicans
Hipler UC, Schröter C, Wollina U, Hipler B.
Article
Bioluminescence Resonance Energy Transfer (BRET)
[120 - 9.110]
Simultaneous dual emission detection for fast kinetic BRET assays
Orban M.
BMG LABTECH 2003
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
Article
[118 - 9.100]
BRET assays performed on the FLUOstar OPTIMA
Orban M.
BMG LABTECH 2002
BRET is an advanced cell-based assay technology for proteomics, including receptor research and the mapping of signal transduction pathways.
Application Note PDF
Nephelometry
[174 - 5.120]
Nephelometric Monitoring Growth of Candida albicans Using BMG LABTECH's NEPHELOstar
U.-C. Hipler
BMG LABTECH 2008
Article
[133 - 5.110]
Measurement of Rheumatoid Factor by NEPHELOstar Microplate Reader
Mack L, Barton A
BMG LABTECH 2005
Article
[125 - 5.106]
Monitoring of Microbial Growth Curves by Laser Nephelometry
Angersbach S, Brand S, Hipler B, Rückert C.
BMG LABTECH 2004
Article
[117 - 5.101]
A fully automated solubility screen in 384-well plate format using nephelometry
Green C, McKee S, Saunders K.
BMG LABTECH 2003
Article
[114 - 5.005]
Nephelometric Test for Determination of Colostrum Quality
AgResearch, BMG LABTECH Australia
Article


AlphaLISA and AlphaScreen are registered trademarks of PerkinElmer, Inc.

Jump to top of page
BMG LABTECH GmbH, Hanns-Martin-Schleyer-Str. 10, D-77656 Offenburg/Germany
Phone: +49 781 96968-0, Fax +49 781 96968-67, Impressum
Copyright ©1995-2009 All Rights Reserved.


Home | Sitemap | Bookmark | Login | Disclaimer | JAPAN
BMG Labtech
Send or print page Send or print page

 

Search Applications Dot

Microplate Readers dot

Support Dot

Contact Dot

Sign up for our newsletter