SNP Genotyping

November 01, 2012

Single Nucleotide Polymorphisms (SNPs) can be used as genetic markers in Agrigenomics to identify seeds or plants in breeding programs.

Dr. Edward Dell | BMG LABTECH
Dr. EJ Dell
PhD, Sales Manager Northwest
BMG LABTECH USA

There are several different chemistries that can perform SNP Genotyping amongst which are KASPar®, Taqman®, and Invader®. These chemistries rely on the detection of multiple fluorophores for each sample and when screening thousands of samples an instrument is needed that can reliably read these multiple fluorophores in a high-throughput manner.

 

The PHERAstar Plus and the PHERAstar FS are the perfect instruments to handle these screens. Having Dual Emission Detection and the ability to read 1536-well microplates in less than a minute, the PHERAstar microplate readers make SNP Genotyping a snap.

 

For more information, check out this application note showing how the misread rate of SNP Genotyping using KASPar® is less than 0.1, while a recent publication shows how the PHERAstar and KASPar® have been used for the genetic mapping of Pigeonpea and Legumes.

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