Under normal reading conditions, most plate readers take a single measurement in the center of each well. For cell based assays where the cell distribution is uneven a single center well reading is insufficient. In some cases, a single center point reading may completely miss the material of interest in a large well format.
To overcome this problem, BMG LABTECH has added an advanced Well Scanning feature to its plate reader lines. In Well Scanning mode the microplate reader can take multiple measurements in each well with up to 30 x 30 data points. Well scanning (30 x 30 matrix) and Direct Optic Bottom reading provide the PHERAstar FS with an excellent platform for advanced high resolution cell-based analysis in 384 well plates.
The software is able to display each scan point graphically and create a map for each well. The user can remove individual scan points or entire sections of each well map with a few mouse clicks. The graphical interface uses multiple color options to display data quickly in a number of different formats.
Another possible way to measure non-homogeneous well contents is BMG LABTECH’s unique Orbital Averaging feature. Using this mode, the plate reader takes several measurements on a defined orbit, collects the data and calculates an average for each well.
The Well Scanning and Orbital Averaging features enable the cell researcher to quantify results without the need to destroy live cells or damage cultures that have taken days or weeks to prepare.