Reagent Injectors

Many of today’s most popular assays (e.g. enzyme kinetics, calcium flux, dual luciferase, and ORAC) require quick reads and the ability to monitor a signal during and after the addition of a reagent. Some of these assays produce signals that decay very quickly after reagent addition, becoming unreadable in only a few seconds. Without the ability to read and inject simultaneously, the user can lose valuable signal information. It is  even possible to read and perform two injections at the same time.

BMG LABTECH’s reader line, with a few exceptions, can be equipped with software controlled reagent injectors. The injector module is fully integrated into the reader platform and is not a bulky add-on. This feature enables delivery of a variety of reagents including buffers, substrates, agonists, and even enzymes into any plate format from 6-384 wells. Options in the control software include injection timing, injection speed, delivery volume, and the ability to inject different volumes in each well of a plate. The control software also allows for multiple additions from the same injector into the same well. Most importantly, though, is the ability of the injectors to deliver reagent simultaneously while reading.

Simultaneous injection and reading ensure that users experience no loss of data and save valuable laboratory time normally consumed by a user adding multiple reagents. The reagent injectors are easily cleaned, chemically inert, and can be configured with various syringe types depending on the customer’s need. High volume syringes are available for large volume buffer delivery and low volume syringes are available for precious, expensive, or temperature sensitive reagents.

 

	In this tutorial from the video training series, you can see a demonstration of a simple software set up for a FLUOstar Omega microplate reader in order to perform kinetic fluorescence measurements of samples in a 96-well plate format. Kinetic fluorescence intensity measurements from top can be followed in real time before, during and after the injection event.
	This video from BMG LABTECH shows how to create and execute a simple kinetic fluorescence protocol including baseline monitoring, reagent injection and continued monitoring of the changing fluorescence intensity signal using BMG LABTECH's Omega series of microplate readers.