Path Length Correction

For many assays such as DNA quantification and protein determination, users frequently compare absorbance results from a microplate reader with those from a 1 cm path length cuvette spectrophotometer.

All microplate readers from BMG LABTECH capable of reading absorbance have a 1 cm path length correction built in via an internal fitting algorithm. Using this correction technique, the 1 cm data from a microplate assay is given with no calculations or estimations made by the user. The path length correction feature can be turned on or off via software as your needs dictate.

A linear regression comparison of data from a BMG LABTECH microplate reader and 1 cm cuvette spectrophotometer produces an r² value of over 0.99 in the UV and visible portions of the spectrum.