Multi-mode microplate readers are capable of operating in two or more detection modes. Typically, they combine an absorbance plate reader, a luminescence plate reader, and a fluorescence plate reader. In addition, the range can be enhanced with more advanced detection modes like TRF, TR-FRET, fluorescence polarization and AlphaScreen/AlphaLISA.
An absorbance microplate reader detects the light photons absorbed by a sample present in a microplate, when exposed to light at a specific wavelength. Compared to a spectrophotometer that measures one sample at a time in a cuvette, an absorbance microplate reader can measure the same assay in higher throughput.
A fluorescence plate reader detects the light emitted by a fluorescent sample present in a microplate upon excitation with light at a specific wavelength. Fluorescence plate readers are used for fluorescence intensity and FRET detection and are available as single- or multi-mode readers, when combined with absorbance and/or luminescence.
A luminescence microplate reader detects the light emitted by chemical, bio-chemical or enzymatic reactions. Luminescence plate readers are used for flash, glow assays and BRET detection and are available as single- or multi-mode readers, when combined with absorbance and/or fluorescence.
A nephelometer microplate reader is an instrument dedicated to the measurement of the turbidity of a sample, usually caused by the presence of insoluble particles suspended in a liquid. Nephelometer microplate readers measure the light scattering generated by these particles and are used for solubility or aggregation studies.
"Most common absorbance-, luminescence- and fluorescence-based assays are able to be run without having to hunt high and low"
"We need to get consistent, high-quality data regardless of location […] for that reason, we chose the PHERAstar FSX"
"The PHERAstar is a very high-end instrument which is very good for high-throughput screening and is the most sensitive instrument I have tested for BRET."
"The CLARIOstar shows a fantastic performance with fluorescence, luminescence and UV measurements […] We are therefore very satisfied with this device and we strongly recommend it"
"The CLARIOstar microplate reader is essential to all the research we do […] Just love it!"