Will the CLARIOstar be able to perform fast kinetic assays?
Indeed, preliminary results with the CLARIOstar have shown it is capable of performing Dual Luciferase Reporter assays. DLR assays are commonly used to assess regulation of gene expression and employ the successive activation of firefly and Renilla luciferase. Included in the reagents that activate Renilla is a reagent that stops the firefly reaction so that Renilla signal can be detected. The CLARIOstar with on-board injectors performed this assay admirably well.