Why does a spectrometer-based microplate reader capture a full absorbance spectrum much faster than a monochromator-based instrument?

May 14, 2012

BMG LABTECH’s spectrometer-based instruments, which includes the Omega Series, SPECTROstar Nano, and PHERAstar FS, can capture a full UV to Visible absorbance spectrum (220-1000 nm) in less than one second per well.

Image of Dr EJ Dell
Dr EJ Dell
PhD, Sales Manager Northwest
BMG LABTECH USA

In comparison, monochromator-based microplate readers take tens of seconds or even minutes to create a full spectrum. One reason is because BMG LABTECH’s spectrometer-based microplate readers are newer technology and thus, in this instance, the newer technology has features that the older technology does not. But more specifically, the BMG LABTECH spectrometer-based instruments use a CCD-camera that captures the whole spectrum of a sample at once,  unlike a monochromator that only captures a narrow wavelength band that has to be ‘built’ into a spectrum. This is why a spectrometer-based instrument can capture a full spectrum at a greater resolution and a greater speed than a monochromator-based instrument.

 

For more information on the UV/Vis absorbance spectrometer technology used in BMG LABTECH's instruments, visit our technology page.

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