What are the most common ELISA conjugate/substrate combinations and what are their appropriate absorbance wavelengths?
May 28, 2012
|Enzyme Conjugate label||Substrate||Reading wavelength (nm) - Kinetic or Nonstopped||Reading wavelength (nm) - End Point or Stopped|
|Horseradish peroxidase (HRP)||OPD (o-Phenylenediamine)||450||492|
|ABTS ((2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid])||414||414|
|Alkaline Phosphatase (Alk-phos)||pNPP (p-Nitrophenyl Phosphate)||405||405|
|β-Galactosidase (β-gal)||ONPG (o-Nitrophenyl-ß-D-galactopyranosidase)||420||420|
Here you can find a list of BMG LABTECH instruments that can perform absorbance measurements including ELISA.