What are the most common ELISA conjugate/substrate combinations and what are their appropriate absorbance wavelengths?
May 28, 2012
| Enzyme Conjugate label | Substrate | Reading wavelength (nm) - Kinetic or Nonstopped | Reading wavelength (nm) - End Point or Stopped |
| Horseradish peroxidase (HRP) | OPD (o-Phenylenediamine) | 450 | 492 |
| TMB (3,3′,5,5′-Tetramethylbenzidine) | 630 | 450 | |
| ABTS ((2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]) | 414 | 414 | |
| Alkaline Phosphatase (Alk-phos) | pNPP (p-Nitrophenyl Phosphate) | 405 | 405 |
| β-Galactosidase (β-gal) | ONPG (o-Nitrophenyl-ß-D-galactopyranosidase) | 420 | 420 |
| Urease | Urea bromocresol | 590 | 590 |
Here you can find a list of BMG LABTECH instruments that can perform absorbance measurements including ELISA.
