Using the SPECTROstar Nano for a wide variety of applications to futher research on dariy cows
Streptococcus uberis is a major pathogen associated with dairy cow mastitis, which is the inflammation of the mammary glands. Mastitis is one of the most economically important infectious diseases affecting the dairy industry, causing up to $50 billion/year in loss of milk production. In this research, Dr. Ripp proposes to isolate different environmental and clinical S. uberis strains from a new dairy facility pre- and post-arrival of cow herds.
The virulence and pathogenicity characteristics will be compared to existing samples of clinical S. uberis strains. The hypothesis is that S. uberis clinical isolates have virulence factors that allow them to more effectively cause mastitis that are not present in the environmental isolates. Dr. Ripp proposes to use the many different features of the SPECTROstar Nano in several different types of experiments, including:
- Bacterial growth kinetic absorbance measurements
- OD spectrum measurement (480 – 600 nm) of individual microtiter plate wells (designed to validate and improve existing non-standardized OD assay measurements)
- Low volume, high-throughput DNA quantification using the LVis plate
- ELISA assays
- Gas vent for CO2 atmospheric control
- Incubation (various temperatures)
- Use of 24 and 96-well plates
- Robotic integration with a Hudson PlateCrane.
As Dr. Ripp notes, “The availability of the SPECTROstar Nano instrument will not only significantly accelerate and simplify data acquisition, but will also allow for new experimental assays to be performed not otherwise possible with our existing instrumentation."