Under what circumstances would I need bottom reading?
Fluorescence calcium signaling (FURA-2, FLUO-3/4/8, Indo, etc.) and transcription assays (beta-lactamase, GeneBLAzer®, etc.) all need sensitive bottom reading to be properly executed. In addition, assays dealing with reporter proteins, like GFP/CFP or YFP need bottom reading capability to provide best results.
A bottom read measurement can also be used in kinetic assays that require an injection such as calcium flux or ORAC.
For more information on direct optic bottom reading visit our technology page.