Under what circumstances would I need bottom reading?

March 05, 2012

A bottom read measurement is usually used in cell based experiments when a lid is needed to prevent contamination or when a plate is sealed to prevent evaporation.

Dr. Edward Dell | BMG LABTECH
Dr. EJ Dell
PhD, Sales Manager Northwest
BMG LABTECH USA

Fluorescence calcium signaling (FURA-2, FLUO-3/4/8, Indo, etc.) and transcription assays (beta-lactamase, GeneBLAzer®, etc.) all need sensitive bottom reading to be properly executed. In addition, assays dealing with reporter proteins, like GFP/CFP or YFP need bottom reading capability to provide best results.


A bottom read measurement can also be used in kinetic assays that require an injection such as calcium flux or ORAC.


For more information on direct optic bottom reading visit our technology page.

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