Transcreener ADP2 FI assay

Transcreener® assays rely on direct, highly-specific detection of nucleotides using antibodies that are able to differentiate between nucleotides on the basis of a single phosphate group. These assays can be used across entire families of nucleotide-dependent enzymes. The Transcreener assays use a homogenous, competitive immunoassay format in which the antibodies are paired with high affinity fluorescent tracers. Displacement of the tracer by the nucleotide being detected causes a change in its fluorescence properties.

The Transcreener® ADP2 Fluorescent Intensity (FI) assay from BellBrook Labs extends the Transcreener platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs (such as the CLARIOstar® and Omega series) as well as more complex plate readers more commonly used in core facilities and HTS labs (such as the PHERAstar® FSX)

The Transcreener® ADP2 FI Assay is a red, competitive fluorescence intensity assay based on the detection of ADP and therefore is compatible with any enzyme class that produces ADP, including protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases and glutamine synthetase. It is a simple one step homogenous detection assay, and is flexible with regard to ATP concentration (0.1 to 1,000 µM ATP). The assay provides excellent signal at low substrate conversion, with a Z' ≥ 0.7 at 2.5% ATP conversion using 1 µM ATP.

Application Notes

For more information about the Transcreener® ADP2 FI Assay and other Transcreener® technologies visit: http://www.bellbrooklabs.com

 

Transcreener is a patented technology of BellBrook Labs.

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