Determine ECA and OCR
The Mitochondrial Stress Test (Cayman Chemical #Item No. 601450) allows you to acquire the extracellular acidification (ECA) and oxygen consumption rate (OCR). To facilitate the measurement you can download the following tools that are compatible with your CLARIOstar®.
The script leads you through the settings of oxygen and carbon dioxide concentration and regulates your ACU accordingly.
The protocol defines your measurement settings. It is designed to measure both: the pH-XtraTM pH-sensitive probe and MitoXpress® Xtra oxygen-sensitive probe in an interval of 4:40 min. After 12 measurements the control compound Oligomycin is injected that increases ECA. After 25 measurements, the control compound FCCP is injected which increases the OCR.
The template calculates the lifetime of the oxygen probe and uses it to calculate amount of oxygen present in your sample and the oxygen consumption rate OCR. It furthermore determines the lifetime of the pH-sensitive probe and uses it to calculate the extracellular pH and the extracellular acidification rate ECA.
Download script, test protocol and analysis template