SNP genotyping using the Amplifluor system and the FLUOstar
SNPs are a common occurring mutation found in DNA sequences. These genetic variations can occur in coding or non-coding regions and can either have an extreme or no effect on a particular organism. Therefore SNP detection has become important for medical research and pharmacology.
The detection of SNPs (SNP Genotyping) can be achieved with a number of different methods. In application note 187 a homogeneous fluorescence based method on PCR is described. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of a FLUOstar Omega from BMG LABTECH.
See application note 187 to learn more about this SNP assay or go to Millipore's website see a more detailed description of this assay.