Parasitology
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Resources
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
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Assay development for essential enzyme activity in the tegument of live Schistosomes
Madhu Sundaraneedi (1) , Luke Becker (2) , Giovanni Abbenante (3) , Alex Loukas (2) , Grant Collins (1) , Mark Pearson (2), (1) School of Physical, Environmental and Mathematical Sciences, University of New South Wales, Australia , (2) Australian Institute for Tropical Health and Medicine, James Cook University, Australia , (3) BMG LABTECH Australia, 12/2017 - 235
Three assays in one well: antimalarial compound library screening
Sheena McGowan, Department of Biochemistry and Molecular Biology , Monash University, 03/2013 - 214
Quantitive, high-throughput, fluorescent-based bioassay to detect Schistosoma viability
Emily Peak , Iain W. Chalmers , Karl F. Hoffmann, Aberystwyth University, 03/2011
Synthesis and Structure-Activity Relationship of Dual-Stage Antimalarial Pyrazolo[3,4-b]pyridines
Read articleJournal of medicinal chemistry
Glucantime-loaded electrospun core-shell nanofibers composed of poly(ethylene oxide)/gelatin-poly(vinyl alcohol)/chitosan as dressing for cutaneous leishmaniasis
Read articleInt. J. Biol. Macromol.
Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development
Read articlePLoS Pathog.
Comprehensive analysis of the secreted proteome of adult Necator americanus hookworms
Read articlePLoS Negl Trop Dis
Evaluation of NanoLuc, RedLuc and Luc2 as bioluminescent reporters in a cutaneous leishmaniasis model
Read articleActa Trop.