Defining physiological normoxia in cultured cells for translation to animal models and man
Learn how the CLARIOstar with ACU replicates in vivo oxygen conditions to increase the significance of your data
Cell-based assays are typically run at non-physiologically relevant O2 levels such as 21%. The speakers show the impact that working under physiological conditions can have to improve the interpretation of data. They present a case study employing a Cardiovascular Endothelial line in which Nrf-2 activity is investigated using ROS and cell metabolism measurements.
The scientific talk explores how and why O2 as a physiological parameter can influence data. The interpretation of disease models will be demonstrated and researchers learn how to adopt a similar strategy to more accurately replicate the in vivo condition on the bench.