Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Novel aggregation-specific fluorogen monitors prefibrillar protein aggregation by fluorescence polarisation (FP)Manjeet Kumar (1) , Yuning Hong (2,3) , David C. Thorn (1) , Heath Ecroyd (4) , John A. Carver (1), (1) Research School of Chemistry, The Australian National University, Australia , (2) Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Australia , (3) School of Chemistry, The University of Melbourne, Australia , (4) School of Biological Sciences, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, Australia, 07/2018
Detection of human tau protein aggregationDelphine Jaga (1) , Franka Maurer (2), (1) Cisbio , (2) BMG LABTECH, 09/2015
Following Abeta fibrillization/aggregation in real-time using a FLUOstar Omega microplate readerFrank Baumann, Hertie Institute for Clinical Brain Research, 12/2014
Real-time quaking induced conversion assay for prion seedingMaggie Nakamura, BMG LABTECH, 12/2012
Use of a BMG LABTECH microplate reader to monitor amyloid formationSarah Shammas , Ann-Christin Brorsson, Department of Chemistry , University of Cambridge, 01/2008
Amyloid formation of fish β-parvalbumin involves primary nucleation triggered by disulfide-bridged protein dimersRead article
Proc Natl Acad Sci U S A
Seeded fibrils of the germline variant of human λ-III immunoglobulin light chain FOR005 have a similar core as patient fibrils with reduced stabilityRead article
J Biol Chem
TDP-43 and Tau Oligomers in Alzheimer\'s Disease, Amyotrophic Lateral Sclerosis, and Frontotemporal DementiaRead article
Self-Replication of Prion Protein Fragment 89-230 Amyloid Fibrils Accelerated by Prion Protein Fragment 107-143 AggregatesRead article
Int J Mol Sci
Kinetic fingerprints differentiate the mechanisms of action of anti-Aβ antibodiesRead article
Nat Struct Mol Biol