Nucleic acid quantification
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
DNA quantification using absorbance (A260) and fluorescent methods (Qubit™ and Quant-iT™/PicoGreen™)Andrea Krumm, BMG LABTECH GmbH, 77799 Ortenberg, Germany, 10/2020
Qubit™ RNA Quantiﬁcation of in vitro transcribed crRNAs using an Echo liquid handler and the CLARIOstar PlusMichael Andrew Crone (1,2) , Manoja Rasamanikkam (3), (1) Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, SW7 2AZ , (2) UK Dementia Research Institute Care Research u. Technology Centre at Imperial College London , (3) BMG LABTECH, Aylesbury, UK, 04/2020
A protein-based biosensor for mRNAAlexander Cook , Christopher P. Toseland, School of Biosciences, University of Kent, UK, 12/2018
Streamlining Next Generation Sequencing quality management using high throughput fluorometric DNA/RNA quantificationUlrike Bönisch , Laura Arrigoni , Diana Santacruz , Nadia Kress, Deep Sequencing Facility, Max Planck Institute of Immunobiology and Epigenetics Freiburg, 09/2018
Quantifying double-stranded DNA with fluorescent dyes: Hoechst 33258 measured on a FLUOstar OmegaMarco Göttig (1) , Andrea Krumm (2) , Rabea Meyberg (1) , Kristian K. Ullrich (1) , Stefan A. Rensing (1), (1) Plant Cell Biology, Faculty of Biology, University of Marburg , (2) BMG LABTECH GmbH, 08/2018
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