Nanoliter high-throughput screening

February 03, 2012

As 1536-well microplates were introduced to high throughput screening (HTS), adoption was not immediate.

Image of Dr EJ Dell
Dr EJ Dell
PhD, Sales Manager Northwest

Now instruments with 1536-well microplate capability are a mandatory consideration for most HTS workflows. Will a similar pattern hold for 3456-well microplate formats? With BMG LABTECH’s PHERAstar FS, scientists now have the ability and the option to go to smaller volumes.


The PHERAstar FS has the ability to reliably measure not only 1536-well microplates, but also 3456-well plates in primary and secondary cell-based HTS assays. Technological advancements provide the perfect platform to reliably measure smaller volumes. Its advanced optical focusing system has a resolution of hundredths of a nanometer, allowing for precise signal measurements from the top and bottom of the microplate. The direct-optic bottom reading has increased sensitivity for smaller volume and for cell-based assays due to an innovation in optical design. Furthermore, two separate high performance lasers allow for extremely fast and ultra-sensitive measurements in AlphaScreen® and time-resolved fluorescence assays such as HTRF®.


As will be showcased at SLAS 2012, BMG LABTECH and Labcyte will present a new nanoHTS platform, incorporating next generation instrumentation and software for nanoliter-scale high-throughput screening. Using Labcyte’s Echo® to precisely dispense nanoliters of reagents into 1536- and 3456-well microplates, measurements taken with the PHERAstar FS show that assay integrity is maintained when miniaturizing different HTS platforms. This new fully automated nanoHTS platform can screen more compounds faster while lowering the average screening costs. The future of HTS is here, nanoHTS, the ability to go to smaller volumes.

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