New developments in the field of proteomics require higher sensitivity and specificity in determining protein concentration. Fluorescent dyes such as NanoOrange® fulfil these requirements since being capable of measuring down to a concentration of 10 ng/ml in a high volume reaction (2.5 ml). Performing the assay in microplates reduces the reaction volume to 200 µl.
For verification of the microplate-based quantification assay, dilutions of bovine serum albumin (BSA) were measured on a multi-mode reader by BMG LABTECH. The detected fluorescence intensity was linear in a concentration range from 200 ng/ml to 10 µg/ml.
Consequently, the microplate reader enables miniaturization and increased throughput of the NanoOrange® protein quantification assay while being more sensitive than competing absorbance assays.