CLARIOstar – measuring sub-picogram levels of dsDNA

September 26, 2017

The CLARIOstar is the most sensitive Microplate Reader tested for dsDNA Concentration, as shown with Biotium’s new AccuBlueTM NextGen dsDNA Dye.

Image of Dr EJ Dell
Dr EJ Dell
PhD, Sales Manager Northwest
BMG LABTECH USA

The CLARIOstar is the most sensitive Microplate Reader tested for dsDNA Concentration, as shown with Biotium’s new AccuBlueTM NextGen dsDNA Dye.

 

As Genomic DNA sequencing techniques develop, like single cell sequencing, greater sensitivity using lower amounts of DNA is desired. Sensitivity using UV/Vis Absorbance techniques (i.e. 260 nm) is limited by Beer’s law, therefore fluorescence intensity assays like PicoGreen® and Qubit® have arisen to achieve greater sensitivity. But, these dyes all have a similar Limit of Detection (LOD) in the low picogram range.

 

To meet this demand, Biotium out of Freemont, CA, has developed their new AccuBlueTM NextGen dsDNA Dye, which can measure sub-picogram levels of dsDNA, but only on our CLARIOstar microplate reader.

 

Figure: AccuBlueTM NextGen dsDNA detection principle.

 

Figure: Quantification of dsDNA in a 384 well plate (50 uL) showing that a 0.309 pg LOD is reached. Taken from Application note 306.

 

See the chart on Biotium’s Website for the list of instruments that were tested and how the CLARIOstar in 384 well plates using 50 uL gave the best performance out of all of them.

For your future reference, download the new dsDNA concentration application note and see how the CLARIOstar and Biotium’s AccuBlueTM NextGen dsDNA Dye can achieve sub-picogram dsDNA sensitivity.

Find out if the CLARIOstar microplate reader can help with your assay needs as well.

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