CLARIOstar – measuring sub-picogram levels of dsDNA
The CLARIOstar is the most sensitive Microplate Reader tested for dsDNA Concentration, as shown with Biotium’s new AccuBlueTM NextGen dsDNA Dye.
As Genomic DNA sequencing techniques develop, like single cell sequencing, greater sensitivity using lower amounts of DNA is desired. Sensitivity using UV/Vis Absorbance techniques (i.e. 260 nm) is limited by Beer’s law, therefore fluorescence intensity assays like PicoGreen® and Qubit® have arisen to achieve greater sensitivity. But, these dyes all have a similar Limit of Detection (LOD) in the low picogram range.
Figure: AccuBlueTM NextGen dsDNA detection principle.
Figure: Quantification of dsDNA in a 384 well plate (50 uL) showing that a 0.309 pg LOD is reached. Taken from Application note 306.
See the chart on Biotium’s Website for the list of instruments that were tested and how the CLARIOstar in 384 well plates using 50 uL gave the best performance out of all of them.
Find out if the CLARIOstar microplate reader can help with your assay needs as well.
PicoGreen is a registered trademark of Invitrogen.