Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Dual Channel Kinetic assays for detecting ligand bias at GPCRsPaul Tewson (1) , Scott Martinka (1) , Kevin M. Harlen (1) , Thom Hughes (1) , Anne Marie Quinn (1) , Sam R.J. Hoare (2) , Carl Peters (3), (1) Montana Molecular, Bozeman, MT , (2) Pharmechanics, Owego, NY , (3) BMG LABTECH Inc., NC, USA, 11/2019
Monitoring intracellular calcium using fluorescent dyes in a mid-throughput assayThomas P. Keeley , Ron Jacob , Giovanni E. Mann , King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine & Sciences, King’s College London, London SE1 9NH, UK, King’s BHF Centre of Research Excellence, School of Cardiovascular Medicine and Sciences, King’s College London, 03/2019
Calcium retention capacity assay evaluates inhibition of mitochondrial permeability transition poreM. Awais , D. Latawiec , R. Sutton, Liverpool Pancreatitis Research Group, Institute of Translational Medicine, University of Liverpool, UK, 11/2017
Simultaneous detection of GPCR second messengers in living cellsP. Tewson (1) , S. Martinka (1) , S. Tillo (1) , T. Hughes (1) , A.M. Quinn (1) , C. Peters (2), (1) Montana Molecular, Bozeman, MT , (2) BMG LABTECH, Cary, NC, 12/2016
Monitoring receptor ligand binding in living cellsDominik Schelshorn (1) , Franka Maurer (2), (1) Geneva Biotech , (2) BMG LABTECH, 07/2015
The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKARead article
J. Biol. Chem.
Severe Autoinflammatory Manifestations and Antibody Deficiency Due to Novel Hypermorphic PLCG2 MutationsRead article
J. Clin. Immunol.
Structure of human GABAB receptor in an inactive stateRead article
The orphan receptor GPR139 signals via Gq/11 to oppose opioid effectsRead article
J. Biol. Chem.
Quantification of Molecular Interactions in Living Cells in Real Time using a Membrane Protein NanopatternRead article