Reactive oxygen species
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Resources
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
- 322
Cell-based assay detects residual nonsteroidal anti-inflammatory drugs (NSAIDs) in effluent of municipal wastewater treatment
K. Bernhard , C. Stahl , R. Martens , M. Frey, SIZ Zellkulturtechnik, c/o Hochschule Mannheim, Mannheim, Germany, 06/2018 - 310
Mitochondrial oxidant generation follows oxygen deprivation and re-oxygenation
Daniel Pastor-Flores , Tobias Dick, German Cancer Research Center (DKFZ), Division of Redoxregulation (A160), 08/2017 - 262
Chemiluminescence measurement of the generation of reactive oxygen species
Bernd Hipler (1) , Uta-Christina Hipler (2), (1) BMG LABTECH , (2) FSU Jena, 12/2014 - 252
Real-time monitoring of genetically encoded redox probes in mammalian cell monolayers
Daria Eyerina , Bruce Morgan , Tobias P Dick, German Cancer Research Center (DKFZ), 05/2014 - 167
Comparison of thioredoxin activity in cortical neuron and glial cells using a BMG LABTECH microplate reader
Sofia Papadia , Frédéric Léveillé , Giles E Hardingham, Centre for Neuroscience Research , University of Edinburgh, 03/2008
Evidence for NADPH oxidase activation by GPR40 in pancreatic β-cells
Read articleRedox Rep.
Hepatoprotective and antioxidant activities of Dicranopteris linearis leaf extract against paracetamol-induced liver intoxication in rats
Read articlePharm Biol
In Vitro and In Vivo Evidence on the Role of Mitochondrial Impairment as a Mechanism of Lithium-Induced Nephrotoxicity
Read articleBiol Trace Elem Res
Duplication of symbiotic Lysin Motif-receptors predates the evolution of nitrogen-fixing nodule symbiosis
Read articlePlant Physiol.
Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells
Read articleProc. Natl. Acad. Sci. U.S.A.
Recommended Products
