Nucleic acid interactions
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Fluorescence polarization-based RNA synthesis assayStefan Reich , Stephen Cusack, European Molecular Biology Laboratory, 10/2018
Protein-induced fluorescence enhancement detects protein-nucleic acid interactions in microplatesFulneček J. , Valuchová S. , Petrov A.P. , Tripsianes K. , Říha K., Research group Molecular Biology of Plants, Central European Institute of Technology, Masaryk University, Brno, Czech Republic, 10/2017
Novel Copper-Containing Cytotoxic Agents Based on 2-ThioxoimidazolonesRead article
J Med Chem
IRES-targeting small molecule inhibits enterovirus 71 replication via allosteric stabilization of a ternary complexRead article
Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6Read article
Molecular basis for DNA repair synthesis on short gaps by mycobacterial Primase-Polymerase CRead article
hnRNPK recognition of the B motif of Xist and other biological RNAsRead article
Nucleic Acids Res.