174

Nephelometric monitoring growth of Candida albicans using BMG LABTECH's NEPHELOstar Plus

U-C. Hipler Friedrich-Schiller University Jena 08/2008

In contrary to CD-EC the complexed ciclopirox was  less effective (Figure 3). At a concentration of 200 μg/mL of the complex, cells were inhibited and at a concentration of 300 μg/mL of the complex, cells  were killed. The CD-CI complex was also less  effective compared to the free CI that caused cell  death already at 10 μg/mL.


Conclusion

 

This study has proven that laser nephelometry in a  96-well microtiter plate can be used as a method for the rapid determination of the solubility of potential drug compounds.


Laser nephelometry can distinguish between the concentration at which the drug just goes into or just comes out of solution. On the other hand, this technique can be used efficiently for monitoring and evaluating the growth of microorganisms like fungi or bacteria.

Nephelometry measures the forward scattered light when passing solutions. This can be used to determine particles in solution as they increase light-scattering. The particles may be derived from drugs at their limit of solubility or from microbes. Hence, determination of drug-solubility and microbial growth are two main applications for nephelometry.

 

Using a microplate-based nephelometer, the NEPHELOstar® Plus, solubility of the antifungal substances Econazole-nitrate and ciclopirox-olamine each complexed with Cyclodextrins was monitored and showed a higher solubility than their uncomplexed form. The Cyclodextrin-complexes were studied regarding their antifungal activity on Candida albicans and revealed a higher efficiency of the Econazole complex as compared to the ciclopirox complex.

 

This study has proven that laser nephelometry in a 96-well microtiter plate can be used as a method for the rapid determination of the solubility of potential drug compounds.

호환되는 리더

go to top