Mycology & yeasts
Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
High-throughput method for dual assessment of antifungal activity and growth kinetics using a FLUOstar OmegaGordon Ramage , Ryan Quinn , Steve Milligan, Glasgow Dental School , Faculty of Medicine , University of Glasgow, 03/2009
Nephelometric monitoring growth of Candida albicans using BMG LABTECH's NEPHELOstar PlusU-C. Hipler, Friedrich-Schiller University Jena, 08/2008
Fluorescence analysis of reactive oxygen species (ROS) generated by six isolates of Aspergillus fumigatusU.-C. Hipler (1) , U. Wollina (1) , D. Denning (2), (1) Department of Dermatology, Friedrich-Schiller University Jena , (2) Department of Microbiology, University of Manchester, UK, 06/2004
A Novel Secreted Cysteine-Rich Anionic (Sca) Protein from the Citrus Postharvest Pathogen Penicillium digitatum Enhances Virulence and Modulates the Activity of the Antifungal Protein B (AfpB)Read article
J Fungi (Basel)
Genomic instability in an interspecific hybrid of the genus Saccharomyces: a matter of adaptabilityRead article
Acetamiprid Affects Destruxins Production but Its Accumulation in Metarhizium sp. Spores Increases Infection Ability of FungiRead article
Impact of biofilm production by Candida species and antifungal therapy on mortality of patients with candidemiaRead article
Production of a lyophilized ready-to-use yeast killer toxin with possible applications in the wine and food industriesRead article
Int. J. Food Microbiol.