One of the daily routines in a molecular biology laboratory is the determination of the DNA content and purity. A widespread method is to use absorbance at 260 nm and determine impurities at 230 and 280 nm. This can be measured at all spectrometer-based BMG LABTECH plate readers.
This note covers various aspects of nucleic acid quantification: from the theoretical background over the concentration range up to the sample volume. It furthermore shows exemplary DNA quantification measurements performed on a SPECTROstar® Nano.