Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Time Resolved Fluorescence (TRF) immunoassay in 384-well format using a matched antibody pair kit and the PHERAstar FSXJamie Campbell (1) , Oliver Carney (2) , , (1) Abcam, Cambridge, UK , (2) BMG LABTECH, Aylesbury, UK, 12/2020
Binding kinetics: high throughput assay for kinase inhibitorsKrumm A (1) , Georgi V (2) , Schiele F (2) , Fernández-Montalván A (2), (1) BMG LABTECH GmbH , (2) Bayer AG, Drug Discovery, Pharmaceuticals, Berlin, Germany, 06/2020
PHERAstar FSX certified for Transcreener assaysFranka Maurer (1) , Meera Kumar (2), (1) BMG LABTECH , (2) BellBrook Labs, 02/2016
Detection of tyrosine kinase activity in AlphaScreen modeFranka Ganske , Marjan Orban, BMG LABTECH, 12/2014
LanthaScreen TR-FRET tyrosine kinase and protein kinase C assayRandy Hoffman (1) , Megan Buros (1) , Kevin Kupcho (1) , EJ Dell (2), (1) Invitrogen , (2) BMG LABTECH, 12/2014
Selective Degradation of GSPT1 by Cereblon Modulators Identified via a Focused Combinatorial LibraryRead article
ACS Chem. Biol.
Allosteric activation of proto-oncogene kinase Src by GPCR-beta-arrestin complexesRead article
J. Biol. Chem.
Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular proteinRead article
Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid ArthritisRead article
J Immunol Res
Engineering of a Potent, Long-Acting NPY2R Agonist for Combination with a GLP-1R Agonist as a Multi-Hormonal Treatment for ObesityRead article
J. Med. Chem.