THUNDER cell signaling assays performed on the CLARIOstar Plus
In this tutorial, Jaime Padrós of BioAuxilium Research introduces a new line of ready-to-use cell signaling assay kits based on their enhanced proprietary TR-FRET platform, THUNDER™.
Jaime discusses immunoassays enabling easy, rapid, sensitive and robust quantification of endogenous proteins in either cell lysates or supernatants with only one reagent addition step.
Moreover, selected cytokine and phospho-protein assays are validated on the CLARIOstar Plus microplate reader. These data demonstrate the superior sensitivity of this plate reader for TR-FRET based assays.
Get more information about THUNDER™ cellular kinase assays in AN361: Analysis of ERK1/2, p38αβγ and STAT3 phosphorylation with the THUNDER™ TR-FRET cellular kinase immunoassays.
In the application note Excellent assay performance of THUNDER™ TR-FRET cell-based cytokine assays performed on the PHERAstar FSX, it is shown how the PHERAstar FSX with TRF laser exhibits much higher sensitivity and dynamic range than a competing HTS microplate reader.