PHERAstar^® FSX は、最高感度と最速測定を実現したハイスループットスクリーニング用マルチプレートリーダーです。独自のオプティックモジュールとデュアル検出器、フルスペクトル吸光度・ALPHAテクノロジー用オプションと、更に高感度になったレーザー光源でのTR-FRETオプションによって、3456well プレートまでのアッセイをストレスなく実現できます。

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Detection Modes

PHERAstar FSX

PHERAstar FSXは取り扱いが簡単なオプティックモジュールで波長の選択をします。モジュールごとにフィルター情報を含んだバーコードがついているので、フィルターの管理も簡単です。その組み合わせはBMGアプリケーションサポートがアッセイに最適なフィルター・ダイクロイックミラー・偏光フィルターなどをセレクトしますので、研究者の方がフィルターの組み合わせに頭を悩ますことはありません。さらに、 PHERAstar FSXは２組４つのPMTを搭載し、測定モードに合わせて自動的に切り替わります。これにより、すべての測定モードにおいて完全二波長同時測定ができるのです。

HTSに対応するために、PHERAstar FSXはロボットシステムへのインテグレーションツールとマルチユーザーコントロールを標準装備しています。

Seven certifications of BMG LABTECH microplate reader: HTRF, DLR, LanthaScreen, Transcreener ADP2 FI, FP, TR-FRET, MycoAlert (from left to right)

特徴

最高感度の蛍光測定・偏光蛍光測定
５つの専用検出器を備えた唯一のリーダー
すべての測定モードで完全二波長同時測定（Alphaテクノロジーを含む）
発光測定でダイナミックレンジ９桁
Alphaテクノロジー（AlphaScreen^®/AlphaLISA^®/AlphaPlex™）専用レーザー光源搭載
高感度TRFレーザー搭載
上方＆下方双方からの最適測定ポイントの調整
試薬注入と検出を同時に行う高精度インジェクター（2chまで)
高速スペクトル吸光度測定

PHERAstar FSX

Z-height focus

Automatic Z-height adjustment is extremely important for obtaining the best possible signal from every sample. If the focus is inaccurate by as little as 0.2 mm, this can result in a signal degradation of up to 25%. BMG LABTECH's Z-height focus adjustment tool scans the whole sample with 0.1 mm resolution and determines the height at which the strongest signal can be measured.

Schematic description of a Dual Emission module of the PHERAstar FSX microplate reader from BMG LABTECH

Simultaneous Dual Emission

Simultaneous Dual Emission (SDE) detection allows the measurement of two emitted wavelengths at the same time. It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to be read once.

Alpha Technology Laser

Excitation lasers significantly improve performance and lower limits of detection, since they yield higher excitation energy at a specific wavelength when compared to xenon lamps.

Both the CLARIOstar and the PHERAstar FSX can be equipped with a laser dedicated to Alpha Technology (AlphaScreen^®, AlphaLISA^® and AlphaPlex^TM) detection. This dedicated laser outperforms any xenon-based light source by specifically exciting donors at 680 nm.

Direct optic bottom reading

Fluorescent and luminescent detection of adherent cells substantially profits from measurements performed from the bottom of the well. The PHERAstar FSX, CLARIOstar Plus and VANTAstar have a free air optical path to the bottom of the microplate, improving sensitivity and eliminating the need for fiber optics or light guides.

Unlike conventional microplate readers, all BMG LABTECH readers require no displacement or installation of any additional hardware when switching between top and bottom reading modes.

Automation

Automation is a key feature in high-throughput screening. BMG LABTECH microplate readers have been successfully integrated into various robotic systems. Small footprint, multiple robotic software interfaces and an automation-friendly plate carrier guarantee an easy integration into all leading robotic platforms. All plate readers can be controlled through ActiveX or DDE interfaces. Moreover, the ASCII data output is the most versatile in the industry and works with most LIMS and automation platforms.

Decay Curve Monitoring

A unique feature of the PHERAstar FSX, Decay Curve Monitoring provides an indispensable tool for assay troubleshooting and performance fine-tuning for TRF, TR-FRET and AlphaScreen^® assays. Thanks to special detectors with direct photon measurement system, fluorophore decay curves can be collected for one or two wavelengths with a user-selectable resolution of down to 2 µs.

While other TR-FRET microplate readers simply give an integration value for the area under the curve, the PHERAstar FSX gives much more!

Open PHERAstar showing pumps for reagent injection

Reagent injectors

Many of today's most popular assays such as enzyme kinetics, calcium flux, and dual luciferase require the ability to monitor a signal during and after the addition of a reagent. All BMG LABTECH readers, with the exception of the SPECTROstar Nano, can be equipped with up to two reagent injectors. Injectors enable reagent delivery to any plate format from 6 to 384 wells.

Optic modules

PHERAstar Optic Modules free users from worrying about which filter is installed, which dichroic is being used, or if the components are installed correctly. Optic Modules incorporate all the parts necessary to run a specific assay. They are labelled with a barcode and detected by the plate reader, eliminating the possibility of using the wrong filter set. Just drop a module into the PHERAstar and read the assay. It is that simple.

Path length correction

Absorbance measurements such as DNA quantification or protein determination detected in a microplate reader are frequently compared and/or normalized to results from a 1 cm path length cuvette. All BMG LABTECH microplate readers have the ability to perform a 1 cm path length correction thanks to an internal fitting algorithm. Using this correction, data normalization to 1 cm path is given with only one mouse click.

Incubation and shaking

Shaking and incubation allow users to mix the well content and easily run measurements at elevated temperatures. The incubator controls the air temperature in the measurement chamber from room temperature up to 45°C, 60°C or 65°C. BMG LABTECH microplate readers are equipped with orbital, double-orbital or linear shaking with definable intensity, duration and direction of motion. These features are available on all BMG LABTECH plate readers.