Viruses are equally a threat to plants, bacteria, animals, and humans. They use their hosts to reproduce and can thereby damage them. This can lead, for example, to crop or farm animal losses and pandemics. On the other hand, viruses serve as tools for genetic engineering and the targeted modification of genomes.
Modern virology characterises viruses molecularly and functionally and uses this information to develop diagnostic tests, antiviral drugs and vaccines. Traditionally, virology largely relied on microscopic methods. Nowadays, microplate-based assays increase throughput and enable the measurement of replication, virus neutralization, binding of molecules to viral particles and much more.
Virus assays range from simple ELISA assays for measuring antibody titer to live-cell assays to measure replication. The variety of virus assays in combination with the need for cell-based methods requires a flexible microplate reader.
The CLARIOstar®Plus microplate reader offers this flexibility. It is a modular multi-mode reader that can be equipped with fluorescence, luminescence, absorbance and advanced detection modes. With its Atmospheric Control Unit, it is further optimized for live-cell assays as it creates the optimal environment for long-term cell-based experiments. The CLARIOstar Plus can be equipped with a red-shifted PMT for increased sensitivity with fluorophores emitting in the red range of light. These are often used in cell assays to avoid autofluorescence.
The PHERAstar FSX multi-mode microplate reader is the ideal platform for screening departments, where potential anti-viral compounds have to be detected quickly and efficiently in high throughput. In addition, it can quickly and effortlessly measure all FRET, TR-FRET and fluorescence polarization dual emission assays. These are often used in binding/interaction assays for anti-viral compound screens.
Browse our Resources section for information about specific applications, literature citations, videos, blog articles and many other publications. Many of the resources provided are associated with current and previous instrument models and versions.
Studying the molecular mechanism of viral replication in real time using the CLARIOstar Plus with ACUMarko Noerenberg (1) , Vincenzo Ruscica (2) , Alfredo Castello (1,2*), (1) MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, Scotland (UK) , (2) Department of Biochemistry, University of Oxford, South Parks road, OX1 3QU, Oxford, (UK) *Correspondence to: firstname.lastname@example.org, 11/2020
Dual Luciferase Reporter (DLR) assay certificationEJ Dell , Carl Peters, BMG LABTECH, 12/2014
Looking for a strong promoter for Physcomitrella patensLucas Schneider (1) , Manuel Hiss (1) , Stefanie Tintelnot (2) , Stefan Rensing (1), (1) University of Marburg , (2) BMG LABTECH, 11/2014
Simultaneous dual emission detection of luciferase reporter assaysMegan Dobbs (1) , Douglas Hughes (1) , Janaki Narahari (1) , Jae Choi (1) , Georgyi Los (1) , Brian Webb (1) , Eric Matthews (2) , Carl Peters (2), (1) Thermo Fisher Scientific , (2) BMG LABTECH, 03/2013
Multiplex analysis of inflammatory cytokines from primary human macrophages using a FLUOstar OmegaBrenda E. Mutch , Kate Willetts , Emma Timms , Brian MJ Foxwell, Kennedy Institute of Rheumatology, 12/2008
Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical libraryRead article
mTOR-dependent translation amplifies microglia priming in aging miceRead article
J Clin Invest
The Cell Envelope Stress Response of Bacillus subtilis towards Laspartomycin CRead article
Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagyRead article
Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP+-Induced Toxicity via Progesterone Receptor SignalingRead article
Oxid Med Cell Longev