DLR assay system
The Dual-Luciferase® Reporter (DLR™) assay system from Promega Corp. provides a luminescent reporter assay to study gene transcription and regulation. The activities of two luciferases are measured sequentially from a single sample reporting the transcription of the gene of interest and a transfection control.
Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors, such as in the Omega series, CLARIOstar® and PHERAstar® FSX microplate readers. In the DLR™ assay system, both reporters yield linear assays with attomole sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.
Fig. 1: Dual Luciferase Reaction - Luciferase Assay Reagent II (LAR II) is injected in the first step and the Firefly reaction is started. Stop and Glo® buffer is injected in the second step, which quenches the Firefly reaction and initiates the Renilla reaction
BMG LABTECH has published Application notes in cooperation with Promega that demonstrate the use of DLR™ technology with BMG LABTECH microplate readers.