Identification and validation of novel pattern recognition receptors as potential therapeutic targets for treating infectious diseases
The long-term objective of this project is to unravel the network of extracellular matrix (ECM) derived Pattern Recognition receptors (PRRs) of monocytes and macrophages and their relevance for function, in order to identify promising new targets for drugs or vaccines for the fight against infectious diseases. Successful completion of the proposed project will provide a comprehensive compendium of all ECM proteins expressed and secreted by monocytes and macrophages within the context of infectious diseases. The proposed project strictly demands high-throughput screening technology to be feasible. The SPECTROstar Nano microplate reader will be an integral element for the proposed experiments, which include ELISA determination of ECMs in monocytes and macrophages, as well as colorimetric phagocytosis assays involving siRNA mediated knockdowns of ECMs.